Spanwise flexibility is a key factor influencing propulsion performance of pectoral foils. Performances of bionic fish with oscillating pectoral foils can be enhanced by properly selecting the spanwise flexibility. The influence law of spanwise flexibility on thrust generation and propulsion efficiency of a rectangular hydro-foil is discussed. Series foils constructed by the two-component silicon rubber are developed. NACA0015 shape of chordwise cross-section is employed. The foils are strengthened by fin rays of different rigidity to realize variant spanwise rigidity and almost the same chordwise flexibility. Experiments on a towing platform developed are carried out at low Reynolds numbers of 10 000, 15 000, and 20 000 and Strouhal numbers from 0.1 to 1. The following experimental results are achieved: (1) The average forward thrust increases with the St number increased; (2) Certain degree of spanwise flexibility is beneficial to the forward thrust generation, but the thrust gap is not large for the fins of different spanwise rigidity; (3) The fin of the maximal spanwise flexibility owns the highest propulsion efficiency; (4) Effect of the Reynolds number on the propulsion efficiency is significant. The experimental results can be utilized as a reference in deciding the spanwise flexibility of bionic pectoral fins in designing of robotic fish prototype propelled by flapping-wing.