＜正＞This talk will focus on our recent work on single-molecule study of signaling protein in living cell membra...
Xiaohong Fang Beijing National Laboratory for Molecular Sciences,Key Laboratory of Molecular Nanostructures and Nanotechnology,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190
Fluorescence imaging of single molecules is becoming a powerful tool to examine biological processes at the molecular level.Using total internal reflection fluorescence microscopy (TIRFM),it has been possible to study the dynamic behavior of single molecules on living cell membranes.Herein,we briefly review the application of TIRFM-based single-molecule imaging in studies of membrane receptors involved in signal transduction.Furthermore,we discuss several examples of our own research on growth factor receptors,including TGF-β receptors,HER2,and EGFR,and speculate possible applications of this technique to investigate other cellular events occurring on or near the plasma-membrane.
