OBJECTIVE Using bronchial asthma rats,to observe the effects of Smooth wheezing effect of polysaccharide.In addition,to study the effects of COM in different bronchial asthma model.METHODS Bronchial rats established by ovalbumin(OVA),were randomly divided into different group.Every other week building reference literature intraperitoneal injection of OVA,21 d after injection of 3 consecutive ultrasonic atomization inhalation of 1% OVA stimulating 30 d,stimulate the 31 d began to medicine.Lavage for 4 weeks ELISA test ratio of IgE,SP-A,IL-4 and IL-5 was serum and bronchoal veolar lavage fluid etc.Data was in x±s tabular format,SPSS16.0 statistics software is used to perform statistical analysis on the data,and P<0.05 shows meaningful statistical difference.RESULTS Quince polysac.charide can reduce the IgE,IL-4 level and elevated the SP-A,IL-5 level in serum and bronchoal veolar lavage fluid.CONCLUSION Quince polysaccharide has antiasthmatic effect on bronchial asthma rats.
Aim To study the activity and mechanism of inhibition of myocardial hypertrophy of total flavonoids of Cydonia oblonga Mill. in spontaneously hypertensive rats. Methods Total flavonoids of COM （COMF） were sepa- rated and purified by the optimal process. SHtl were divided into 6 groups： SHR control group （SHR） , captopril group （SHR ＋ CAP, 25 μg · g^-1）, Eucommia ulmoides Oliver group （SHR ＋ EUO, 30 μg · g^-1）, low （SHR ＋ COMF-L, 40 μg · g^-1） ,middle （SHR ＋ COMF-M, 80 μg· g^-1） and high dose （SHR ＋ COMF-H, 160 μg · g^-1） of COMF groups. Wistar-Kyoto （WKY） rats （n= 8 ） were given distilled water as control The drugs were given by intragastric administration for 16 weeks. The histological and pathological examination of the heart were performed and organic damage were valued. The levels of Ang II and ALD in blood and heart were evaluated. The mRNA and protein expression of ACE, ACE2 and AT~ was determined by RT-PCR and Western blot to evaluate the effect of COMF on RAAS. Results Compared with SHR control group, HW, HW/BW, LVM and LVM/BW de- creased in SHR ＋ COMF-M and SHR ＋ COMF-H groups; Cadiomyocyte hypertrophy was inhibited in COMF groups; The concentration of Ang 11 and ALD in heart and blood decreased; ACE and AT1 mRNA and protein ex- pression in heart tissue decreased while ACE2 mRNA expression increased （P 〈 0.01 or P 〈 0.05） , Conclusion Total flavonoids of Cydonia oblonga Mill. showed the effect of inhibition of myocardial hypertrophy in spontaneously hypertensive rats and the mechanism was related to. inhibit the activity of Renin-angiotensin-aldosterone system.
