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国家教育部博士点基金(2004)

作品数:3 被引量:4H指数:2
相关作者:陈海王艳艳崔莉莉孙善全余维华更多>>
相关机构:哈尔滨医科大学重庆医科大学齐齐哈尔医学院更多>>
发文基金:国家教育部博士点基金国家自然科学基金黑龙江省研究生创新科研项目更多>>
相关领域:医药卫生更多>>

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孕期高蛋白膳食对子代大鼠解偶联蛋白和肉毒碱棕榈酰基转移酶的影响被引量:2
2008年
目的探讨孕期膳食对子代肥胖和肥胖相关基因解偶联蛋白(UCP)和肉毒碱棕榈酰基转移酶1(CPT1)表达的影响,寻找肥胖预防和治疗的靶标,寻求合理的孕期膳食。方法将Wistar孕鼠按随机数字表法分为两组,孕期全程分别食用标准膳食(SD)和高蛋白质膳食(HPD)。所产雄性子鼠分别为对照组(CON)和高蛋白质组(HP),均由CON组的母亲喂养,24d断乳,用SD喂养至成年后,将CON组大鼠按随机数字表法分为两组:一组继续食用SD(CON);另一组食用高脂膳食(HFD),为高脂对照组(HFCON)。同时HP组也食用HFD,HFCON和HP组高脂诱导肥胖至实验结束。分期处死大鼠,检测血清甘油三酯(TG)和褐色脂肪中UCP2、UCP3及CPT1mRNA的表达丰度。结果孕期HPD可降低子代断乳前后的体重和高脂诱导肥胖后的体重及肥胖率;断乳时和高脂诱导肥胖后HP组的血清TG分别低于CON和HFCON组;HP组UCP3和CPT1的表达持续高于CON和HFCON组,UCP2的表达在断乳后也持续高于CON和HFCON组;动态观察发现断乳时和高脂诱导肥胖后CPT1的表达均明显升高,高脂诱导肥胖后UCP2、UCP3的表达也明显升高;但在食用SD至成年时CPT1的表达较断乳时略有下降,UCP3的表达与断乳时基本一致。结论孕期适当增加蛋白质摄人可降低子代成年高脂诱导肥胖发生的风险;程序性升高子代UCP2、UCP3和CPT1基因的表达;UCP2、UCP3和CPT1可通过参与脂肪酸氧化调节肥胖的发生。
董艳梅孙长颢崔莉莉王艳艳
关键词:肥胖解偶联蛋白
水通道蛋白5在大鼠大脑组织中的分布及表达被引量:2
2008年
目的:观察水通道蛋白5(AQP5)在大鼠脑组织中的分布和表达,为研究脑组织中水分子的运输和平衡机制提供形态学基础。方法:运用免疫组织化学和免疫荧光技术,观察正常成年Wistar大鼠脑组织中AQP5的分布;运用免疫印迹观察AQP5的表达,并与AQP4在脑组织的分布和表达进行比较。结果:AQP5分布于大脑皮质的软脑膜、脉络丛、血管周围、海马锥体细胞层、齿状回颗粒细胞层、视上核、视交叉上核内和大脑纵裂两侧皮质深部,与AQP4分布范围相似;除此以外,AQP5还独自分布于大脑皮质下神经细胞。免疫印迹显示AQP5表达明显弱于AQP4。结论:AQP5在大鼠脑组织中分布广泛,可能协同AQP4在脑组织水运输平衡,脑脊液产生与回流及渗透压的调节过程中,发挥重要作用。
杨美孙善全汪克建刘辉余维华陈海邱国平
关键词:水通道蛋白大脑
Evaluation of postural stability in patients wit unilateral vestibular hypofunction: effect of feet orientation
2008年
Objective To investigate effect of feet orientation on the evaluation of the postural stability in patients with unilateral vestibular hypofunction(UVH) by timed standing tests and static posturography (SPG). Methods 65 subjects with UVH and 92 healthy subjects regarded as control group took the postural stability tests in four different stances including(1) standard Romberg test, (2) feet-apart stance test, (3) tandem and (4) unilateral standing tests. In each stance, the postural stability was measured in both eyes open (EO) and eyes closed(EC) conditions. The average time that subjects kept balance before falling in each test conditions was recorded by stopwatch as the timed result. In addition, the sway velocity(SV) of center of foot pressure in the upright stance during standard Romberg test and feet apart stance, regarded as postural stability, was also recorded as SPG. Results (1) The balance-maintaining time of the UVH group in tandem and unilateral standing with EO and EC was decreased(P < 0.001) compared with the control group. (2) The SVs during standard Romberg test and foot-apart stance with EO were not different between the UVH group and control group (P = 0.118 and 0.110 respectively), but significant with EC condition(P < 0.001). (3) For both groups, the SV during foot-apart standing was shorter than standard Romberg test both with EO and EC(P < 0.05 and P < 0.001). (4) There was no correlation between the balance time and SV for either groups(P > 0.05). Conclusions The results suggest that the tandem and unilateral stance tests may provide additional information about the upright stance to the SPG measurement in patients with UVH. The effect of feet orientation on SPG measurements should be considered.
LIU Bo1, KONG Wei-Jia1, LENG Yang-ming1 Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
关键词:VERTIGO
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