研究强磁重力环境对人成骨细胞MG-63中发动蛋白-2表达和分布的影响。采用大梯度超导磁体模拟空间重力环境,该超导磁体可以提供3种不同的强磁重力环境(high magnetic gravitational environment,HMGE),即0g(12T),1g(16T)和2g(12T)。将MG-63细胞分别在0g(12T)、1g(16T)、2g(12T)及对照环境(1g,地磁)中培养24h后,采用Real Time PCR、Western印迹以及激光扫描共聚焦显微术等方法检测HMGE对发动蛋白-2的表达及定位的影响。结果显示,与对照组相比,0g(12T)、1g(16T)、2g(12T)环境处理组MG-63细胞中发动蛋白-2在mRNA上的表达量分别上调6.43、15.57、1.29倍;在蛋白质水平上,0g(12T)、1g(16T)环境处理组细胞发动蛋白-2分别上调89%和7%,而2g(12T)环境处理组则下调41%;在对照组中发动蛋白-2弥散分布于细胞质中,而0g(12T)处理组发动蛋白-2则有从细胞边缘向核周集中的趋势。上述结果说明强磁重力环境影响了发动蛋白-2在MG-63细胞中的表达和定位。
With the development of superconducting technology, the superconducting magnet, which can produce a stable large gradient high magnetic field (LG-HMF) environment to
UAi-Rong Qian, Wei Zhang, Xiang Gao, Li-Fang Hu, Rui Meng and Peng Shang Institute of Special Environmental Biophysics, Key Laboratory for Space Biosciences &Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University Xi’an, 710072, China
＜正＞With the development of superconducting technology,the superconducting magnet,which can produce a stable la...
Ai-Rong Qian,Wei Zhang,Xiang Gao,Li-Fang Hu,Rui Meng and Peng Shang Institute of Special Environmental Biophysics,Key Laboratory for Space Biosciences &Biotechnology,Faculty of Life Sciences,Northwestern Polytechnical University Xi’an,710072,China
Superconducting magnet provides a long time and stable high gradient magnetic field environment,which is appli...
Airong Qian Wei Zhang Zongcheng Tian Shengmeng Di Lifang Hu Pengfei Yang Yuanyuan Weng Peng Shang Key Laboratory for Space Bioscience and Biotechnology
