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国家自然科学基金(51272246)

作品数:5 被引量:16H指数:2
相关作者:朱立新耿小平许小亮田开亮赵红川更多>>
相关机构:中国科学技术大学安徽医科大学第一附属医院更多>>
发文基金:国家自然科学基金更多>>
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Ordered silicon nanorod arrays with controllable geometry and robust hydrophobicity
2015年
Highly ordered silicon nanorod(Si NR)arrays with controllable geometry are fabricated via nanosphere lithography and metal-assisted chemical etching.It is demonstrated that the key to achieving a high-quality metal mask is to construct a non-close-packed template that can be removed with negligible damage to the mask.Hydrophobicity of Si NR arrays of different geometries is also studied.It is shown that the nanorod structures are effectively quasi-hydrophobic with a contact angle as high as 142°,which would be useful in self-cleaning nanorod-based device applications.
王子文蔡家琦吴以治王会杰许小亮
关键词:纳米棒纳米球
Bio-compatibility and cytotoxicity studies of water-soluble CuInS_2-ZnS-AFP fluorescence probe in liver cancer cells被引量:5
2016年
BACKGROUND: The oncogenesis of hepatocellular carcinoma(HCC) is not clear. The current methods of the pertinent studies are not precise and sensitive. The present study was to use liver cancer cell line to explore the bio-compatibility and cytotoxicity of ternary quantum dots(QDs) probe and to evaluate the possible application of QDs in HCC.METHODS: CuInS_2-ZnS-AFP fluorescence probe was designed and synthesized to label the liver cancer cell HepG 2. The cytotoxicity of CuInS_2-ZnS-AFP probe was evaluated by MTT experiments and flow cytometry. RESULTS: The labeling experiments indicated that CuInS_2-ZnS QDs conjugated with AFP antibody could enter HepG 2 cells effectively and emit intensive yellow fluorescence by ultraviolet excitation without changing cellular morphology. Toxicity tests suggested that the cytotoxicity of CuInS_2-ZnS-AFP probe was significantly lower than that of CdT e-ZnS-AFP probe(t test, F=0.8, T=-69.326, P<0.001). For CuInS_2-ZnS-AFP probe, timeeffect relationship was presented in intermediate concentration(>20%) groups(P<0.05) and dose-effect relationship was presented in almost all of the groups(P<0.05). CONCLUSION: CuInS_2-ZnS-AFP QDs probe had better biocompatibility and lower cytotoxicity compared with CdT e-ZnS-AFP probe, and could be used for imaging the living cells in vitro.
Ming-Ya YangJian HongYan ZhangZhen GaoTong-Tong JiangJiang-Luqi SongXiao-Liang XuLi-Xin Zhu
关键词:剂量-效应关系流式细胞仪
免疫金纳米笼对人乳腺癌细胞靶向光热治疗作用被引量:2
2014年
目的基于金纳米笼的光热转换特性,观察经抗体修饰的免疫金纳米笼对人乳腺癌细胞MCF-7的靶向光热效应,探讨其对肿瘤细胞的杀伤效果及其细胞毒性。方法由银(Ag)立方纳米晶与次氯金酸(HAuCl4)置换反应得到金纳米笼,经Anti-CK19抗体修饰后制成具有生物靶向性的免疫金纳米笼。实验组(A组):将金纳米笼-抗体探针链接细胞后,由功率密度为1.5 W/cm2、波长为808 nm的激光照射30min;对照组(B组)分为2组:BⅠ组经相同光照强度和波长的激光单纯光照30 min;BⅡ组为单纯加金纳米笼无光照。观察A、B组温度随时间变化情况,通过荧光终止剂细胞染色及流式细胞术检测免疫金纳米笼的光热杀伤效应,应用四甲基偶氮唑蓝(MTT)比色法观察其细胞毒性。结果 A组温度升高明显,达48℃,而B组温度无明显变化,维持在35℃左右。荧光终止剂细胞染色及流式细胞术显示A组细胞大量凋亡,而B组细胞活性基本未受影响(P<0.05)。MMT法显示细胞毒性较小(P<0.05)。结论免疫金纳米笼对MCF-7细胞具有靶向杀伤作用,被用于光热治疗。
田开亮朱立新许小亮尹乃强黄乾鹏赵红川耿小平
关键词:光热治疗乳腺肿瘤
单切口腹腔镜与传统腹腔镜行胆囊切除术的疗效比较:Meta分析被引量:10
2013年
目的:评价单切口腹腔镜胆囊切除术(SILC)与传统腹腔镜胆囊切除术(CLC)的临床疗效。方法:计算机检索Cochrane图书馆、MEDLINE、EMBASE、Pubmed等数据库,查找发表于2010-2013年相关随机对照试验文献进行资料提取、质量评价和Meta分析。结果:共纳入16个研究1 300例患者,研究发现与CLC相比,SILC在出血量、疼痛、美观效果方面有优势,在住院时间、中转开腹率方面无差异,手术时间、并发症方面存在劣势。结论:SILC在美观等方面可以达到更好的效果,但是也存在一定的缺点,如手术时间长;总之,两种术式各有长短,需酌情选择。
黄乾鹏朱立新许小亮耿小平
关键词:胆囊切除术腹腔镜单切口META分析
Extraordinary optical transmission through a subwavelength composite hole-pillar array
2014年
We numerically investigate the transmission properties of a subwavelength composite hole-pillar array. As the radius of the pillar increases, the transmission properties experience a complex evolution. It is found that the magnetic dipole resonance of the pillar suppresses the surface plasmon polariton resonance(SPPR) at the gold-air interface. There are two strong transmission peaks associated with the magnetic dipole resonance of pillar and SPPR at the gold-silica interface. A peak associated with magnetic quadrupole resonance of the pillar is observed. Moreover, there is a weak peak associated with the coupling between the whispering-gallery plasmon(WGP) mode and magnetic dipole. Our work is helpful for making a dual band optical filter.
邵伟佳李为民许小亮王会杰吴以治郁菁
关键词:亚波长表面等离子体激元磁偶极子光学滤波器
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