The samples of pueraria lobata ohwi were digested by HNO3 + HClO4.contents of the next trace elements Fe,Zn,Cu,Mn,Ca,Mg,Co,Cr,Ni,Cd,Pb and Al in the bloom,base,stem,and leaf of Impatiens balsamina have been determined by Flame Atomic Absorption method.The result show that the recovery is about 97.5%-103.2%,and the RSD<2.7%,with good accuracy and precision.The order of the content of the trace elements of high to low is as follows:leaf>stem>bloom>base.The importance of essential trace elements Zn,Fe,Mn,Cu for human each has a higher content.In addition,toxic trace elements such as lead,arsenic and cadmium are also found.
To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis(L.)Osbeck and citrus grandis(L.)Osbeck by the way of HPLC.With shimadzu vp-ODS(4.6 mm×150 mm,5 μm) colum and the mobile phase is CH3CN-(H3PO4.pH=3.5)(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable(R>1.5),the linearity rang of hesperidin and naringin are 0.022μg^1.80 μg and 0.023μg^1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.
