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蜂毒肽融合蛋白在裂殖酵母中的表达研究: 将抗菌肽之一的蜂毒溶血肽(Melittin)基因序列定向克隆到表达载体pESP-2中,构建GST-Melittin融合蛋白。利用载体上严格受硫胺素浓度调控的启动子Pnmtl,实现融合蛋白在裂殖酵母Schizosaccha...; 胡宗利刘志昭邓磊潘宇陈国平; 关键词：蜂毒肽裂殖酵母蛋白表达; 文献传递

Silencing LeSGR1 gene in tomato inhibits chlorophyll degradation and exhibits a stay-green phenotype: The full-length cDNA of LeSGR1 was cloned from tomato by RT-PCR and RACE.The cDNA encoded a protein of 272 ami...; Zongli Hu 1,Lei Deng 1,Bo Yan 1,Yu Pan 1,Min Luo 1,Xuqing Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College, Chongqing University,Chongqing 400044; 关键词：TOMATO STAY-GREEN SENESCENCE; 文献传递

水稻中一个谷胱甘肽转移酶基因的克隆、表达和酶活性分析(英文)被引量：1: 2008年; OsGSTL1是位于水稻3号染色体上的一个λ类谷胱甘肽转移酶基因,由8个内含子和9个外显子组成,编码一个由243个氨基酸组成的多肽链。将OsGSTL1克隆到酵母表达载体pYTV上,转化大肠杆菌,然后再转化酵母菌PEP4。Western印迹分析表明外源OsGSTL1基因在转基因酵母中表达,分析半乳糖诱导表达的酵母粗提液的谷胱甘肽转移酶活性表明:转基因酵母的谷胱甘肽转移酶较非转基因酵母和未诱导的酵母高,说明OsGSTL1在转基因酵母中受半乳糖的诱导表达,具有谷胱甘肽转移酶活性。; 胡廷章黄小云肖国生屈霄霄; 关键词：谷胱甘肽转移酶水稻

Co-suppression of the EIN2-homology Gene LeEIN2 Inhibits Fruit Ripening and Reduces Ethylene Sensitivity in Tomato: EIN2(ethylene insensitive 2) is a very important component in ethylene signal transduction pathway. Recently,t...; Zongli Hu 1,Lei Deng 1,Xuqing Chen 1; 关键词：CO-SUPPRESSION ETHYLENE TOMATO; 文献传递

One-Step Purification of Functional Light-harvesting 2 Complexes from Rhodobacter sphaeroides: Most common method for isolation of light-harvesting 2 complexes(LH2) is very time-consuming. Functional LH2 c...; Zhiping Zhao,Zongli Hu,Yan Liang,Tingzhang Hu,Yun Tu,Guoping Chen (Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University,Chongqing 400044); 关键词：HIS-TAG; 文献传递

Cloning and Functional analysis of LeMBF1 involved in abiotic and biotic stress response in tomato: The full-length cDNA of LeMBF1 was cloned from tomato by RT-PCR.The cDNA encoding an open reading frame of 139...; Zongli Hu 1,Lei Deng 1,Dafei Wang 1,Yu Pan 1,Yang Liu 1,Xuqing.Chen 2,Tingzhang Hu 1 and Guoping Chen 1 (1 Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering college, Chongqing University,Chongqing 400044; 关键词：TOMATO; 文献传递

番茄脂氧合酶基因的表达和酶活性分析被引量：2: 2011年; 将番茄脂氧合酶基因Tom loxD克隆至酵母表达载体pPIC9K中,转化毕赤酵母GS115菌株,构建组成型表达Tom loxD的酵母工程菌株。SDS-PAGE和W estern blot分析表明,经甲醇诱导,Tom loxD蛋白在酵母中得到表达,表达的融合蛋白分子量约为103.56 kD,与预测的分子量一致。酶活性分析表明,酵母中表达的Tom loxD蛋白具有脂氧合酶活性。半定量RT-PCR分析表明,Tom loxD基因在番茄中表达受机械损伤的诱导,损伤处理的番茄叶片中脂氧合酶的活性明显增高。说明Tom loxD基因在番茄防御信号中发挥作用。; 胡廷章肖国生陈再刚石汝杰吴应梅; 关键词：脂氧合酶番茄脂氧合酶活性毕赤酵母

植物脂肪氧化酶的研究进展被引量：26: 2009年; 植物脂肪氧化酶(LOX)是一个多基因家族,是由单一的多肽链组成的含有非血红素铁、不含硫的过氧化物酶。LOX催化具有顺,顺-1,4-戊二烯结构的多元不饱和脂肪酸的双加氧反应。植物中,不同脂肪氧化酶的最适pH、pI、底物和产物特异性、时空表达特性、亚细胞定位等存在差异。LOX参与的生理过程涉及损伤、病原攻击、种子萌芽、果实熟化、植物衰老、脱落酸和茉莉酸合成,LOX也在正常的植物生长和生殖生长过程中作为营养储藏蛋白,参与脂类迁移、响应营养胁迫、调节"源"与"库"的分配。对LOX家族的深入理解,将有助于LOX在作物新品种的选育、新型植保素的开发、食品加工等方面得到广泛的应用。; 胡廷章胡宗利屈霄霄任彦荣陈国平; 关键词：脂肪氧化酶基因表达亚细胞定位

A Powerful Hybrid puc Operon Promoter Tightly Regulated by Both of IPTG and Low Oxygen Level: Rhodobacter sphaeroides has been intensively studied and provides an excellent model for studying both photosy...; Zongli Hu1,Zhiping Zhao1,Yu Pan,Yun Tu,Guoping Chen (Key Laboratory of Biorheological Science and Technology,Ministry of Education,Bioengineering College,Chongqing University,Chongqing 400044); 关键词：IPTG; 文献传递

辣椒离体再生及遗传转化研究进展被引量：3: 2011年; 离体再生技术与遗传转化技术促进了传统农作物育种技术的发展并定向改良了辣椒性状.但由于辣椒较其它茄科植物再生困难,导致在利用DNA重组技术改良辣椒对生物和非生物胁迫抗性时增加了难度.近年来,辣椒器官再生、花药培养、胚培养和细胞培养等离体培养技术取得了巨大的成果.就辣椒离体培养及相关生物技术进行了综述,并指出了存在的问题并对其应用前景进行展望.; 曾华胡宗利陈国平胡廷章; 关键词：辣椒离体再生

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国家自然科学基金(30771464)