Objective:To investigate the relationship of poly(ADP-ribose)glycohydrolase(PARG) with poly(ADP-ribose) polymerase(PARP),vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(b-FGF) in colorectal carcinoma(CRC).Methods:Immunohistochemical analysis was used to detect PARG,PARP,VEGF and b-FGF in human colorectal carcinoma.Flow cytometry was used to detect PARG and PARP in murine CT26 cell line.Gallotannin(GLTN) was served as PARG inhibitor.Results:The individual positive rates of PARG,PARP,VEGF and b-FGF were 55.81%(24/43),97.67%(42/43),79.07%(34/43) and 81.40%(35/43),respectively,which were significantly higher than those of control group.The positive PARG was correlated to PARP(r=0.3703,P<0.05) and b-FGF(r=0.4838,P<0.05).The positive PARP was correlated to VEGF(r=0.3968,P<0.05) and b-FGF(r=0.5610,P<0.05).Both PARG and PARP were expressed in CT26 cells.The positive staining rates of PARG and PARP in GLTN-treated group were 7.3% and 52.38%,respectively.They were markedly reduced than those of control group(55.41% and 95.28%,P<0.01,n=10000).Conclusion:The data suggest that PARG expression probably plays a role for VEGF and b-FGF expression in colorectal carcinoma.
Objective:To investigate the significance of Poly(ADP-ribose) glycolhydrolase(PARG) and membrane type 1 matrix metalloproteinase(MT1-MMP) expressions in human colorectal carcinoma.Methods:Immunohistochemical staining for PARG and MT1-MMP was carried out on colorectal adenoma-carcinoma tissue microarrays containing normal colorectal mucosae,adenoma,adenoma with malignant transformation and adenocarcinoma(total 130 specimens).The expressions of PARG and MT1-MMP in the GLTN [Gallotannin]-treated and GLTN-untreated lovo cells were detected by Western Blot.Results:PARG expression in adenocarcinoma(83.1%) and adenoma with malignant transformation(66.7%) was significantly higher than that in normal colorectal mucosa(10%) and adenoma(10.5%).Expression of MT1-MMp in normal colorectal mucosa and adenoma was negative,while the expression in adenocarcinoma(80.3%) and adenoma with malignant transformation(72.2%) was high.The expressions of PARG and MT1-MMP in adenocarcinoma with metastasis and in late tumor stages were significantly higher than those in adenocarcinoma with no metastasis and in early tumor stages.Thus,PARG expression shows a positive correlation with the expression of MT1-MMP.The expressions of PARG and MT1-MMP in GLTN-treated lovo cells were weaker than that in GLTN-untreated lovo cells.Conclusion:The expression of PARG was probably related to the development of colorectal carcinoma.PARG may play an important role for the regulation of MT1-MMP expression in colorectal carcinoma.
Xian LiGuang-jie DuanYa-lan WangN. Jasmine FauzeeQiao-zhuan Li
