OBJECTIVE:To observe the effect of the Traditional Chinese Medicine,modified Taohongsiwu decoction,versus pyridoxine on patients with hand-foot syndrome(HFS)from capecitabine,sorafenib,and gefitinib chemotherapy for gastric,lung,breast,colon,or rectal cancer.Also,to compare quality of life of patients in each group.METHODS:Patients were assigned randomly to group A or B.Group A was given modifiedTaohongsiwu decoction to soak hands and feet for 30 min,once daily.Group B was given 100 mg pyridoxine orally,twice daily.After a 2-week treatment,thetherapeutic effect was assessed by observing three major symptoms,including pain,ulceration,and muscular atrophy.This was assessed with the HFS-14 questionnaire.RESULTS:Significant differences were observed between the two groups in pain relief,and improvement of daily life,walking,and interpersonal communication(P<0.01).No significant differences in driving ability or interpersonal relationships were found.After 2 weeks,the effective rate was 88.3%in group A,which was significantly higher than the50%in group B(P=0.00).CONCLUSION:Modified Taohongsiwu decoction is effective in the treatment of patients with HFS.It improves patients'quality of life according to the HFS-14.
Objective:To observe the anti-virus effects of andrographolide(AD) on the retinoic acid-inducible gene-l(RIG-l)-like receptors(RLRs) signaling pathway when immunological cells were infected with H1N1.Methods:Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation,and immunological cells were harvested after cytokines stimulation.Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line(16HBE).The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium(MTT) assay.After the virus infected cell model was established,AD was added into the medium as a treatment intervention.After 24-h co-culture,cell supernatant was collected for interferon gamma(IFN-γ) and inteiieukin-4(IL-4) enzyme-linked immunosorbent assay(ELISA) detection while immunological cells for real-time polymerase chain reaction(RT-PCR).Results:The optimal concentration of AD for anti-virus effect was 250 μg/mL IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus,RIG-I,IFN-β promoter stimulator-1(IPS-1),interferon regulatory factor(IRF)-γ,IRF-3 and nuclear transcription factor κB(NF-κB) mRNA levels increased significantly(P<0.05).When AD was added into co-culture medium,the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased,RIG-I,IPS-1,IRF-γ,IRF-3 and NFk B decreased significantly in each group with significant statistic differences(P<0.05).Conclusions:The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality.And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.
