本文应用微分干涉相衬法对优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl和暗褐蝈螽G.sedakovii(Fischer von Waldheim)雄性精巢管基部、输精管、贮精囊和精包,及雌性受精囊中精子束的形态变化进行了观察,对探讨螽斯近缘种的生殖隔离机制和生殖生物学具有重要意义。结果表明:这两种蝈螽的精子束通过精包转移到雌性受精囊后,精子束的形态发生了显著变化。精巢管基部的精子为游离的单个精子;输精管、贮精囊和精包中精子成束排列形成较分散的精子束,精子束头部包裹有粘液帽;雌性受精囊中的精子束的精子呈羽状排列,精子的头部汇集在中央轴上。两种蝈螽精子束形态差异不显著。
通过组织切片和SDS-PAGE方法,研究优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl雄性附腺的结构及分泌蛋白的特性。结果表明,优雅蝈螽雄性附腺由3类腺管组成:乳白长腺管、透明腺管和乳白短腺管,腺管的管壁组织结构相似,从内到外依次为单层上皮细胞、基膜、肌肉层,不同腺管管腔分泌物H-E染色后呈现不同颜色。SDS-PAGE分析各种腺管的分泌蛋白具有特异性。
The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni(Phaneropterinae)was determined.The putative initiation codon for cox1 was TTA.The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood(ML)and Bayesian inference(BI).When all 37 genes(mtDNA)were analyzed simultaneously,the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling.The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses.Rhaphidophoridae to be a sister group of Tettigoniidae,and the relationships among four subfamilies of Tettigoniidae were(Phaneropterinae +(Conocephalinae +(Bradyporinae + Tettigoniinae))).Pyrgomorphidae was the most basal group of Caelifera.The relationships among six acridid subfamilies were(Oedipodinae +(Acridinae +(Gomphocerinae +(Oxyinae +(Calliptaminae + Cyrtacanthacridinae))))).However,we did not recover a monophyletic Grylloidea.Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae.ML and BI analyses of all protein coding genes(using all nucleotide sequence data or ex-cluding the third codon position,and amino acid sequences)revealed a topology identical to that of the entire mtDNA genome dataset.However,22 tRNAs genes excluding the DHU loop and TψC loop(TRNA),and two rRNA genes(RRNA)perform poorly when analyzed as single dataset.Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA.Excluding tRNA genes,rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.
