The experimental study of air-entrained concrete specimens subjected to different cycles of freeze-thaw was completed. The dynamic modulus of elasticity, weight loss, the cubic compressive strength, compressive strength, tensile strength and cleavage strength of air-entrained concrete were measured after 0, 100, 200, 300, 400 cycles of freeze-thaw. The experimental results showed that the dynamic modulus of elasticity and strength decreased as the freeze-thaw was repeated. The influences of freeze-thaw cycles on the mechanical properties, the dynamic modulus of elasticity and weight loss were analyzed according to the experimental results. It can serve as a reference for the maintenance, design and the life prediction of dams, hydraulic structures, offshore structures, concrete roads and bridges in northern cold regions.