The aim of current study was to investigate the chemical constituents from the flowers of Rhododendron molle G. Don. The isolation and purification of components were achieved by a series of chromatography including silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were identified based on 1D, 2D NMR, and mass spectral analysis. Fifteen known compounds were isolated and their structures were identified as 2E,4Z-abscisic acid（1）, 2α-hydroxy-oleanolic acid（2）, oleanic acid（3）, asiatic acid（4）, benzyl glucoside（5）, dibutyl phthalate（6）, β-sitosterol（7）, vitexin（8）, quercetin（9）, steraric acid（10）, rhodomollein I（11）, rhodojaponin VI（12）, rhodomollein XI（13）, rhodojaponin II（14）, kalmanol（15）. Compounds 1–10 were isolated from Rhododendron molle for the first time.
A new triterpenoid saponin （compound 1）, together with a known one （compound 2） was isolated from the roots of Silene rubicunda Franch. The isolation and purification were performed by a series of chromatographic techniques including silica gel, sephadex LH-20, and reversed-phase HPLC. Their structures were elucidated on the basis of combination of mass spectrometry and 1D, 2D NMR spectroscopy, and the new compound was identified as 3-O-{β-D-galactopyranosyl-（1→2）-[β-D-xylopyranosyl- （ 1→3 ）]-β-D-glucuronopyrannosyl } -28-O- { β-D-xylopyranosyl-（ 1 →3 ）-β-D-xylopyranosyl-（ 1 α4）-α-L-rhamnopyranosyl-（1→2）- [β-D-quinovopyranosyl-（1α4）]-β-D-fucopyranosyl} quillaic acid.
In the present study, in order to investigate the chemical constituents of Physal＆ alkekengi L. var. franchetii （Mast.） Makino, the isolation of ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified based on 1D, 2D NMR, and mass spectral analysis, A total of 14 compounds were obtained, and their structures were identified as physalin P （1）, 4,7-didehydroneophysalin B （2）, physalin D （3）, 5α-hydroxy-25,27-dihydro-7- dehydro-7-deoxyneophysalin A （4）, 4,7-didehydrophysalin B （5）, ursolic acid （6）, wogonin （7）, blumenol A （8）, nobiletin （9）, liquiritigenin （10）, schizandrin （11）, 5-hydroxymethylfurfural （12）, 5-（hydroxymethy1）-2-（dimethoxymethyl）furan （13）, 1-O- [3-O-2-methyl-5-（2,3,4-trimethyl）pheny1-2,3-pentanedi]-β-D-xylopyranosyl-（1→6）-β-D-galactopyranoside （14）. Among them, compound 14 is a new compound. Compounds 7-11, 13 are isolated from Physalis alkekengi L. var.franchetii （Mast.） Makino for the first time.
To investigate the phytochemical constituents of aboveground parts of Croton tiglium L. （family Euphorbiaceae）, its ingredients were isolated by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were identified based on 1D, 2D NMR and mass spectral analysis. A total of 10 phorbol esters were obtained. Among them, compound 1 （12-O-（2-methyl）butyryl-4ct-deoxyphorbol-β-isobutyrate） and compound 10 （20-formyl-4a-deoxyphorbol- β-acetate） were two new compounds, and the other eight were known compounds.
Croton crassifolius Geisel belongs to the genus croton of the family euphorbiaceae. By means of solvent extraction and repeated chromatorgraphy on silica gel, Sephadex LH-20 and HPLC, the ethanol extract of the roots of Croton crassifolius Geisel was chemically investigated, which led to the isolation of nine diterpenoids. By using spectroscopic analysis, their structures were identified as follows： Crassifolin P（1）, Crassifolin J（2）, 1,5,6-trimethyl-5-（2-（5-oxo-2,5-dihydrofuran-3-yl）ethyl）-1,2,3,4,5,6,7,8-octahydronaphthalene-1-carboxylate（3）, Penduliflaworosin（4）, isoteucvin（5）, Crassifolin G（6）, teucvin（7）, Chettaphanin I（8）, mallotucin D（9）. Among of them compound 1 was a new compound.
In the present research, in order to study the chemical constituents ofPaliurus ramosissimus （Lour.） Poir., the isolation of its ingredients was performed by repeated chromatography on silica gel, Sephadex LH-20, ODS and preparative HPLC. Their structures were elucidated on the basis of combination of mass spectrometry and 1D, 2D NMR spectroscopy. A total of 8 compounds were obtained, and their structures were identified as 313-hydroxy-27-（3,4-dihydroxybenzoyl）oxylup-20（29）-en- 28-oic acid （1）, betulinic acid （2）, lupeol （3）, 27-O-trans-caffeoylcylicodiscic acid （4）, ceanothic acid （5）, 24-hydroxyceanothic acid （6）, dihydrokaempferol （7）, eriodictyol （8）. Among them, compound 1 was a new compound. Compounds 3-4, 7-8 were isolated from Paliurus ramosissimus （Lour.） Poir. for the first time.
Phytochemical investigations of the aerial parts of Reineckia carnea, collected in Yunnan Province of China, were conducted to explore new chemical constituents. A series of chromatographic and spectroscopic procedures were utilized on the aqueous solution after partitioned with ethyl acetate, which resulted in the separation of a new furostanol-type glycoside and four known compounds. The structures of the isolated compounds were elucidated on the basis of spectroscopic techniques（1D and 2D NMR, IR, HRESIMS） as 26-O-β-D-glucopyranosyl-（25S）-5β-furost-20（22）-en-1α,3β,26-triol-1-O-α-L-arabinopyranosyl-（1→2）-[α-Lrhamnopyranos-yl]-3-O-α-L-rhamnopyranoside（1）,（1β,3β,16β,22S）-cholest-5-en-1,3,16,22-tetrol-1,16-di-（β-D-glucopyranoside）（2）, diosgenin（3）, β-sitosterol（4）, ecdysterone（5）.
