<正>Objective To screen miRNA profiles of malignantly transformed human bronchial epithelial cells,16HBE-T,induced by anti-benzo[a]pyrene-trans-7,8-diol-9,10-epoxide(anti-BPDE),and to analyze putative miR-10a targets in 16HBE-T.Methods A hovel microarray platform was employed to screen miRNA profiles of 16HBE-T cells transformed by anti-BPDE.Microarray data for miR-10a and miR-320 were validated using quantitative real time polymerase chain reaction(QRT-PCR).The expression of a putative target for miR-10a,HOXA1,was analyzed by reverse transcription polymerase chain reaction(RT-PCR) and QRT-PCR.Results In comparison with the vehicle-treated cells(16HBE-N),16HBE-T exhibited difierential expression of 54 miRNAs,in which,45 were over-expressed and 9 were down-regulated.The five most highly expressed miRNAs were miR-494,miR-320,miR-498,miR-129,and miR-106a.The lowest expressed miRNAs were miR-10a,miR-493-5p,and miR-363~*.Three members of miR-17-92 cluster,miR-17-5p,miR-20a,and miR-92,showed significantly higher abundance in 16BHE-T as miR-21,miR-141,miR-27a,miR-27b,miR-16 and miRNAs of the let-7 family.The putative target for miR-10a, HOXA1 mRNAwas up-regulated 3-9-foldin 16HBE-T,as compared with 16HBE-N.Conclusion Thefindings of the study provide information on differentially expressed miRNA in malignant 16HBE-T,and also suggest a potential role of these miRNAs in cell transformation induced by anti-BPDE.HOXA1 is similarly up-regulated,suggesting that miR-10a is associated with the process of HOXA1-mediated
YUE-LAN SHENYI-Guo JIANGANNE R. GREENLEELAN-LAN ZHOULIN-HUA LIU
