Objective： To investigate the effects and mechanisms of Gong-tone music on the immunological function in rats with the Chinese medicine syndrome of Liver （Gan）-qi stagnation and Spleen （Pi）-qi deficiency （LSSD）. Methods： Twenty five male Wistar rats of SPF grade were randomly divided into 5 groups： normal group, model group, Xiaoyao Powder （逍遥散） group, Gong-tone group and combined group （the combination of Gong-tone and Xiaoyao Powder）, with 5 rats in each group. The rat model for the Chinese medicine syndrome of LSSD was induced by chronic bandage and irregular diet. The course of treatment was 21 days. After the treatment, the levels of serum gastrin and IgG were detected by enzyme-linked immunoabsorbent assay （ELISA）. Phagocytosis of macrophages was detected by the neutral red uptake assay and T cell proliferation was investigated by 3-（4,5-dimethylthiazolyl）-2,5-diphenyltetrazolium bromide （MTT） assay. Results： The serum gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in the model group were significantly decreased compared with those in the normal group （P〈0.05）. Compared with those in the model group, the serum levels of gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in Gong-tone, Xiaoyao Powder, and combined groups were significantly increased （P〈0.05）. The combined group was superior to either Gong-tone group or Xiaoyao Powder group. Conclusion： Gong-tone music may upregulate the immunological function and play a role in adjuvant therapy in the Chinese syndrome of LSSD.
