An on-chip electrochemical detector for microfluidic chips was described, based on integrated carbon nanotube (CNT) electrodes directly onto the chip substrate through microwave plasma chemical vapor deposition (MWPCVD). The attractive performance of the integrated CNT electrodes was demonstrated for the amperometric detection of sucrose, glucose and D-fructose. The integrated CNT electrodes showed stronger electrocatalytic activity than gold electrodes.