Objective： To reveal the effect of Jianpi Jiedu recipe （JPJDR） on angiogenesis and the PTEN （Phosphatase and tensinhomolog deleted on chromosome ten）/PI3K/AKT signaling pathway in the course of H. pylori infection-inducedcarcinogenesis of gastric mucosa in C57BL/6 mice. Methods： Two-hundred C57BL/6 mice were randomly divided intofive groups （control group, model group, JPJDR low-dose group, JPJDR medium-dose group, and JPJDR high-dosegroup）, 40 in each group. A mouse model of gastric cancer, induced by H. pylori standard strain infection, wasestablished. The mice of JPJDR low-dose, middle-dose, and high-dose groups were intragastrically administered 250,500, and 1000 mg/kg JPJDR per day, respectively. After 72 weeks, the H. pylori infection in gastric mucosa of the micewas analyzed by rapid urease test; the pathological changes in the gastric mucosa of mice were assessed byhistopathological examination, and micro-vessel density （MVD）, vascular endothelial growth factor （VEGF）, andPTEN/PI3K/AKT levels were determined. Results： The incidence of gastric cancer in each group （control group, modelgroup, JPJDR low-dose, medium-dose, high-dose group） was 0%, 26.3%, 13.2%, 10%, and 7.5% respectively. Theincidence of gastric cancer in the Chinese medicine group was significantly lower than that of the model group （P =0.020, P = 0.023, P = 0.007）. The expression of MVD and VEGF in the model group was significantly higher than thatin the control group （P = 0.002, P 〈 0.001）, while the expression of MVD and VEGF decreased in the Chinese medicinegroup. The expression of p-PTEN and p-AKT in the model group was significantly higher than that in the control group（All P 〈 0.001）, while Chinese medicine could reduce the expression of p-PTEN and p-AKT to varying extents.Conclusion： Long-term infection of C57BL/6 mice with H. pylori induces gastric carcinogenesis, by increasing gastricmucosal MVD, promoting the expression of VEGF, inhibiting the activity of PTEN, and activating the PI3
Ning-Ning LiuWan-Li DengChao-Jun WuYuan-Yuan FengXin-Wen MaQi Li
