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中国博士后科学基金(2012M511817)

作品数:3 被引量:9H指数:2
相关作者:王方华刘亮王永华杨博更多>>
相关机构:华南理工大学更多>>
发文基金:中国博士后科学基金国家自然科学基金中央高校基本科研业务费专项资金更多>>
相关领域:建筑科学生物学更多>>

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产蛋白酶菌株Bacillus subtilis GS-1发酵条件优化及蛋白酶水解应用初步研究被引量:3
2012年
利用Plackett-Burman(PB)试验和相应面优化方法对枯草杆菌GS-1菌株发酵产蛋白酶的条件进行优化,以期获得高活性的蛋白酶发酵液。同时,将发酵所产蛋白酶用于蛋白水解实验,以进一步考察蛋白酶的水解特性。首先,通过PB试验,筛选出3个影响菌株发酵过程中蛋白酶活力的主要因子(氯化铵、初始pH值及培养温度)。其次,通过响应面优化法,进一步探讨各参数之间的相互关系并最终拟合得出发酵产酶活的方程。结果表明,在氯化铵(3.768 g/L),初始pH(5.6)以及培养温度22.4℃的条件下,发酵液中蛋白酶的最高酶活力能达到372.814 U/mL。水解试验结果表明:相对于植物来源的蛋白,该蛋白酶粗提物对于动物来源的蛋白具有较大的水解偏好型。该蛋白酶粗提物对于由谷氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸或精氨酸所组成的化学键有较强的水解偏好性。以上结果表明:该菌株发酵所产的蛋白酶在蛋白水解加工行业中具有潜在的开发应用价值。
王方华刘亮王永华杨博
关键词:发酵优化蛋白水解
Properties and calculation of normal section bearing capacity of RC flexural beam with skin textile reinforcement被引量:2
2013年
In order to overcome the wide crack of ordinary reinforced concrete (RC) at service stage which affects the service performance and durability of structures,a kind of concrete structure with skin textile reinforcement is proposed,namely a part of concrete cover of RC members is replaced by textile reinforced concrete (TRC).The flexural experimental results indicate that when the reinforcement ratios of steel bars are constant,compared with control beams,the average value of crack loads of the beams,whose reinforcement ratios of textile are 0.018%,0.036% and 0.055%,increases by 15.5%,20.4% and 31.1%,respectively,the average value of yield loads respectively increases by 12.5%,19.9% and 21.1% and the average value of ultimate loads respectively increases by 8.5%,26.0% and 44.0%,respectively.Considerable reduction in cracks width and spacing is observed for specimens with a TRC layer,and when the beams yield,the maximum crack width of the beam with textile stuck no sand and the beam with textile stuck sand is reduced by around 60% and 70%,respectively.Surface treatment of textile and mixing polypropylene fiber into fine grained concrete contribute to enhance the service performance of the flexural element.Embedding U-shaped hoop has almost no effect on the control of the crack width.Finally,the calculation method of ultimate bearing capacity of this flexural component with TRC layer was presented.Comparison between the calculated and the experimental values reveals satisfactory agreement,and the maximum error is no more than 6%.
尹世平吕恒林徐世烺
关键词:TEXTILE
盐水干湿循环作用下TRC加固RC受弯梁的研究
试验研究了氯盐干湿循环作用下循环次数、弯曲应力水平、持载方式对TRC加固RC梁受弯性能的影响。试验制作了8根梁:1根不进行加固,作为对比梁;7根用TRC加固。研究表明:在一定干湿循环次数下,因钢筋锈蚀率较低,加固梁的屈服...
尹世平叶桃贾申那明望
关键词:干湿循环TRC受弯性能应力水平
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Improved Mechanical Properties of Textile Reinforced Concrete Thin Plate被引量:4
2013年
Textile reinforced concrete (TRC) is especially suitable for the thin-walled and light-weight structural elements with a high load-bearing capacity. For this thin element, the concrete cover thickness is an important factor in affecting the mechanical and anti-crack performance. Therefore, the influences of the surface treatment of the textile and mixing polypropylene fiber into the concrete on the properties of the components with different cover thickness were experimentally studied with four-point bending tests. The experimental results show that for the components with the same cover thickness, sticking sand on epoxy resin-impregnated textile and adding short fiber into the concrete are helpful to improve their mechanical performance. The 2-3 mm cover thickness is enough to meet the anchorage requirements of the reinforcement fiber and the component has good crack pattern and mechanical behavior at this condition. Comparison between the calculated and the experimental Values of flexural capacity reveals satisfactory agreement. Finally, based on the calculation model of the crack spacing of reinforced concrete structures, the crack extension of this thin-wall component was qualitatively analyzed and the same results with the experimental were obtained.
尹世平徐世烺LI Hedong
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