In this paper,the surface-enhanced Raman scattering(SERS)is used as an analytical tool for the detection and identification of pathogenic bacteria of Escherichia coli(E.coli)and Salmonella typhimurium(S.typhimurium).Compared with normal Raman signal,the intensity of SERS signal is greatly enhanced.After processing all SERS data,the obvious differences between the SERS spectra of two species are determined.And applying the chemometric tools of principal component analysis and hierarchical cluster analysis(PCA-HCA),the SERS spectra of two species are distinguished more accurately.The results indicate that SERS analysis can provide a rapid and sensitive method for the detection of pathogenic bacteria.
乙型肝炎作为一种发病率高、死亡率高的传染性疾病,已严重威胁人类健康,乙肝病毒(hepatitis B virus,HBV)是诱发乙型肝炎的重要病因。目前,最主要的治疗方法是运用抗病毒药物控制病情,但这些药物都不能完全治愈乙型肝炎且复发率高。近年来,RNA干扰技术(RNA interference,RNAi)逐渐成为有效、快速治疗乙型肝炎的新疗法。利用RNA干扰技术体外合成针对HBV基因的siRNA,选择适当的载体将其运送至靶细胞,使HBV基因沉默,从而抑制病毒复制,可有效达到治疗乙肝的效果。本文围绕siRNA沉默HBV基因的设计原理、递送载体、靶向策略、以及治疗效果与应用前景等方面进行了系统综述,为今后siRNA治疗乙肝的临床应用提供参考。
