Natural products(NPs)continue to play a pivotal role in drug discovery programs.The rapid development of synthetic biology has conferred the strategies of NPs production.Synthetic biology is a new engineering discipline that aims to produce desirable products by rationally programming the biological parts and manipulating the pathways.However,there is still a challenge for integrating a heterologous pathway in chassis cells for overproduction purpose due to the limited characterized parts,modules incompatibility,and cell tolerance towards product.Enormous endeavors have been taken for mentioned issues.Herein,in this review,the progresses in naturally discovering novel biological parts and rational design of synthetic biological parts are reviewed,combining with the advanced assembly technologies,pathway engineering,and pathway optimization in global network guidance.The future perspectives are also presented.
The title compound（S）-N-（2-oxoazepan-3-yl）biphenyl-4-carboxamide（C19H20N2O2, Mr = 308.37） was synthesized by the reaction of（S）-α-amino-∑-caprolactam with 4-phenyl-benzoyl chloride. Its chemical structure was determined by 1H NMR, 13 C NMR, H RMS and X-ray single-crystal diffraction. The crystal belongs to the triclinic system, space group P1 with a = 8.2650（17）, b = 13.774（3）, c = 15.109（3） A, β = 90.20（3）o, V = 1645.6（6） A3, Z = 4, Dc = 1.245 g/cm3, μ = 0.081 mm-1, F（000） = 656, R = 0.0756 and w R = 0.1491 for 5964 observed reflections with I 〉 2σ（I）. The title compound was also used to prepare polyamide.
In this study, etherification of ginkgolide B and dimethylaminoethyl chloride hydrochloride was investigated as a model reaction in a micro-flow system （MFS）, providing the resulting ethers in high yield with fewer side effects. Meanwhile, this novel process in MFS worked well for other ginkgolides from Ginkgol biloba and halides, giving moderate yields.
Star-branched polyamide 6 was prepared via anionic ring-opening polymerization of ε-caprolactam in the presence of a simple benzene-centered trifunctional activator of N,N＇,N＂-trimesoyltricaprolactam. A high polymer yields of above 95% were achieved at 160 ℃ for 15 min utilizing ε-caprolactam magnesium bromide as a catalyst. Compared with its linear counterpart, the resultant star-branched polyamide 6 showed smaller relative viscosity （1.51 ）, decreased melting temperature （218 ℃） and lower crystallinity （24.2%）. The specific properties demonstrated the existence of a star-branched structure and provided potential advantages in engineering applications.
