Objective To study the characteristic 2-（2-phenylethyl）chromone components of endophytic fungal strain of Aquilaria sinensis by solid culture. Methods The compounds were isolated by various chromatographic methods such as silica gel, reverse-phase silica gel, Sephadex-LH20 column chromatography as well as crystallization. Results Seven 2-（2-phenylethyl）chromone analogues were isolated from the solid culture of Botryosphaeria rhodina A13. Their structures were established by spectral data as well as physicochemical properties, and identified as 6-hydroxy-7-methoxy-2-（2-phenylethyl）chromone （1）, 6,7-dimethoxy-2-（2-phenylethyl） chromone （2）, （5S,6R,7S,8R）-2-（2-phenylethyl）-5,6,7,8-tetrahydrchromone （3）, 6- hydroxy-2-（2-phenylethyl）chromone （4）, 4＇-hydroxy-2-（2-phenylethyl）chromone （5）, 6-methoxy-2-phenethyl-4H-chromen-4-one （6）, and 6-methoxy-2-（4＇-methoxy-phenethyl）-4H-chromen-4-one （7）. Conclusion All of the compounds are isolated for the first time from the genus Botryosphaeria. This research opens up a new vista to produce the characteristic components of agarwood by endophytic fungi.
Yao ZhangHong-xin LiuWen-shan LiMei-hua TaoQing-ling PanZhang-hua SunWei YeHao-hua LiWei-min Zhang
