Isoangustone A （1） is an isoprenylated flavonoid isolated from licorice. It has been reported to possess anti-microbial, anti-oxidative, anti-inflammatory, and anti-tumor activities. In order to increase its structural diversity, microbial transformation of 1 was conducted by Mucor hiemalis CGMCC 3.14114 to obtain three new compounds. By extensive NMR and MS spectroscopic analyses, their structures were identified as isoangustone A 7-O-glucoside （2）, isoangustone A 7-O-glucoside-4＇-O-sulfate （3）, and isoangustone A 7,3 ＇di-O-glucoside （4）, respectively. The major biotransformation reaction was glycosylation at C-7. Sulfation is rare for microbial transformation.
