A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.
Hydrogel micropatterns of poly(ethylene glycol) and polyacrylamide were prepared with a facile photolithographic method. Monomer solutions containing photoinitiator were directly polymerized by UV illumination through a transparency photomask, forming 2-dimensional gel patterns on silanized glass surfaces. The chemically patterned surfaces thus prepared could be used as the template for patterning mammalian cells and formation of structured droplets.
