Epstein-Barr virus(EBV)is associated with nasopharyngeal carcinoma(NPC)tumorigenesis.However,the mechanism(s)connecting EBV infection and NPC remain unclear.Recently,a new class of EBV microRNAs(miRNAs)has been described.To determine how EBV miRNAs control the expression of host genes,and to understand their potential role in NPC tumorigenesis,we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyngeal epithelial tissues.We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC.Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the carcinogenesis of NPC.A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated,suggesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis.An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis.
目的:探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测多发性骨髓瘤(multiple myeloma,MM)、意义未明单克隆免疫球蛋白增多症(monoclonal gammopathy of undetermined significance,MGUS)及反应性浆细胞增多症(reactive plasmacytosis,RP)的几种常见的异常基因,提高对几种常见浆细胞疾病的诊断及鉴别诊断水平。方法:回顾性分析2012年8月至2015年8月在中南大学湘雅医院初诊的61例MM、20例MGUS及20例RP患者的临床表现、影像学、实验室检查及FISH检测结果。结果:FISH检测61例MM患者中50例患者出现基因异常,FISH阳性检出率为81.9%,其中1q21扩增19例(31.1%)、D13S319缺失18例(29.5%)、RB1缺失10例(16.4%)、IGH易位10例(16.4%)、p53缺失7例(11.4%),出现两种或多种基因异常的阳性率为12例(19.8%);20例MGUS中FISH阳性检出率为30%,其中1q21扩增4例(20%)、IGH易位2例(10%),未发现两种或多种基因异常;而RP患者仅1例出现D13S319缺失,阳性率仅为5%。三组两两比较差异具有统计学意义(P<0.05)。结论:MM患者中FISH的阳性检出率为81.9%,明显高于MGUS及RP的患者,应用FISH可检测MM中的多种异常基因,对MM,MGUS及RP的鉴别诊断、预后判断有重要的临床参考价值。
