Sixteen mungbean lines were analyzed using 56 random primers. Different DNA bands were detected between Bruchid resistant lines and susceptible lines. According to the cluster results, the 16 lines can be divided into four groups, including brucid resistant wild types, resistant cultivated lines, resistant progenies and a mixed group. BSA method was used to identify DNA markers that related with bruchid resistant gene by using resistant line and susceptible line and their F2 progeny. One codominant marker was identified, which generated a fragment of 1.79 kb in resistant lines and 1.03 kb in susceptible lines. Finally, this codominant marker was considered to be tightly linked with bruchid resistant gene and could be useful in resistant germplasm identification and marker-assisted selection.
CHENG Xu-zhenWANG Su-huaWU Shao-yuZHOU Ji-hongWANG Shu-minCharles Y Yang
Bruchid is a serious insect pest of mungbean [Vigna radiata (L.) Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglü 1, from the World Vegetable Center, Asian Vegetable Research and Development Center (AVRDC). Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus (named as Br2). Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.
SUN Lei CHENG Xu-zhen WANG Su-hua WANG Li-xia LIU Chang-you MEI Li XU Ning
