This paper presents a novel experimental design to greatly improve the calibration accuracy of the acceleration-insensitive bias and the acceleration-sensitive bias of the dynamically tuned gyroscopes(DTGs).In order to reduce experimental cost,the D-optimal criteria with constraints are constructed.The turntable positions and the number of test points are chosen to build D-optimal experimental designs.The D-optimal experimental designs are tested by multi-position calibration experiment for tactical-grade DTGs.Test results show that,with the same cost,the fit uncertainty is reduced by about 50% by using the D-optimal 8-position experimental procedure,compared to using a defacto standard experimental procedure in ANSI/IEEE Std 813-1988.Furthermore,the new experimental procedure almost achieves optimal accuracy with only 12-position which is half the cost of the widely adopted 24-position experimental procedure for achieving optimal accuracy.