A wheat pot experiment was conducted under greenhouse conditions to assess the effect of copper contamination on soil nematode diversity by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) method and morphological analysis. The soil was treated with CuSO4·5H2O at the following concentrations:0,50,100,200,400,and 800 mg kg-1 dry soil,and the soil samples were collected at wheat jointing and ripening stages. Nematode diversity index(H') from morphological analysis showed no difference between the control and the treated samples in either of the sampling dates. At the wheat ripening stage,nematode diversity obtained by the PCR-DGGE method decreased noticeably in the Cu800 treatment in comparison with the control. With optimization of the method of nematode DNA extraction,PCR-DGGE could give more information on nematode genera,and the intensity of the bands could reffect the abundance of nematode genera in the assemblage. The PCR-DGGE method proved promising in distinguishing nematode diversity in heavy metal contaminated soil.
WANG Shi-Bin LI Qi LIANG Wen-Ju JIANG Yong JIANG Si-Wei
Soil samples were collected with distance at 5, 20, 40, 80, 160, and 320 m from the Shen-Ha （Shenyang-Harbin） Highway, Northeast China, to investigate the effect of heavy metals of highway origin on soil nematode guilds. The contents of soil Pb, Cu, Zn, and the nematode community structure were analyzed. The results showed that the contents of total and available Pb, Ca, Zn varied significantly with the different distances from the highway. Pb was the main pollutant in the soils in the vicinity of Shen-Ha Highway. The zone from 20 to 40 m away from the highway was the most polluted area. The highest abundance of soil nematodes was found at 5 m while the lowest at 20 m away from the highway. Thirty six genera of nematodes belonging to 23 families were identified. Nematode guilds having different responses to soil heavy metals were classified into four types. Soil nematode guilds may act as a prominent indicator to heavy metal pollution of highway origin.
HAN DechangZHANG XiaokeTOMAR Vijay Vikram SinghLI QiWEN DazhongLIANG Wenju
