利用AFLP分子标记技术,以陆地棉（Gossypium hirsutum L.）品种中棉所8号与海岛棉（Gossypium barbadense L.）品种Pima90杂交产生的F2群体为材料,构建了包含11个连锁群和86个标记位点（31个标记位点前人未曾报道）的连锁图谱,图谱总长562.4 c M,约占棉花基因组的12.5%,标记间平均距离为6.5 c M。该图谱有5个连锁群被定位到相应的染色体上,6个连锁群未定位于染色体上。应用复合区间作图法分析F2单株和F2∶3家系的纤维品质性状,检测到11个与纤维品质有关的QTL,包括5个纤维长度（FL）、3个整齐度（FU）、1个马克隆值（FM）、2个伸长率（FE）的QTL,分别解释表型变异的17.7%～38.3%、16.3%～24.2%、24.7%、22.2%～46.5%。
Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cel- lulose is synthesized. We obtained cDNA of 20―25 d post anthesis (DPA) fiber from 109 F2 progeny and developed a cotton fiber transcriptome profiling via cDNA-AFLP technology using 37 different primer combinations. The F2 population originated from an interspecific cross between Gossypium hirsutum and Gossypium barbadense. One hundred and thirty-eight absence/presence polymorphic transcript- derived fragments (TDFs), with sizes ranging from 100 bp to 722 bp, were screened. Of these, 75 (53.62%) were polymorphic between the parents of the F2 population. Sequencing the 75 transcripts revealed that 37 of them had been reported to be cotton fiber ESTs. Nine of 75 transcript sequences were homologous to 7 cloned cotton fiber genes, encoding cysteine proteinase, vacuolar H+-pyro- phosphatase, vacuolar H+-ATPase, catalytic subunit, arabinogalactan protein, putative receptor protein kinase PERK1, GIA/RGA-like gibberellin response modulator and cellulose synthase. Some other transcripts may represent new gene fragments in cotton fiber development. Surprisingly, 46 of the 75 transcripts were mapped to a single linkage group. The transcriptome groups and the sequenced TDFs could serve as important resources in the functional genomic research of cotton fiber development.
PAN YuXin MA Jun ZHANG GuiYin HAN GaiYing WANG XingFent MA ZhiYingt
