[Objective] This study aimed to investigate the safe dosage of imazethapyr for an imazethapyr resistant millet variety and its effect on weed control. [Method] The Imazethapyr resistant millet ＂M1508＂ was used as the experimental material and the experiment was arranged in a split plot design with two factors： herbicide application time and herbicide dosage. Application time was taken as the main plot with six treatments： A1 （preemergence treatment）, A2 （postemergence treatment on the 3rd d）, A3 （postemergence treatment on the 6th d）, A4 （postemergence treatment on the 9th d）, A5 （postemergence treatment on the 12th d）, A6 （postemergence treatment on the 15th d）; spraying dosage（B） with nine treatments： B1 （0 ml/hm2 , taking water as control）, B2 （750 ml/hm2 ）, B3 （1 125 ml/hm2 ）, B4 （1 500 ml/hm2 ）, B5 （2 250 ml/hm2 ）, B6 （3 000 ml/hm2 ）, B7 （4 500 ml/hm2 ）, B8 （6 000 ml/hm2）, B9 （7 500 ml/hm2 ）; water was added at 675 kg/hm2 . [Result] Imazethapyr showed a good herbicidal effect, and the imazethapyr resistant new variety M1508 also had a high drug resistance; the dosage range of 750 -7 500 ml/hm2 was safe for millet; considering the weed control efficacy, the yield and weeding cost comprehensively, postemergence treatment on the 6th day at 1 500 -2 250 ml/hm2 was the best for herbicide application. [Conclusion] Matched chemical weeding techniques to imazethapyr resistant millet varieties were preliminarily identified, and the efficacy of imazethapyr on weed control and its security on millet were investigated, providing references for the application and promotion of imazethapyr resistant millet varieties.
热激蛋白70（Heat shock protein 70,HSP70）是一类结构保守的分子伴侣类型应激蛋白,在调控动植物新陈代谢和信号传导方面发挥重要作用。本研究以谷子基因组数据库为基础,利用生物信息学方法鉴定了谷子HSP70基因家族成员,并对其序列的保守性、保守基序分布、内含子-外显子结构、在染色体上的分布以及系统发育关系进行了分析。结果表明,谷子HSP70家族是一个至少包含25个成员的多基因家族,基因编码区介于295~851个氨基酸之间;家族成员共有10类保守基序,长度分布于17~50个氨基酸之间;结构分析表明,各成员的内含子数目位于0~8之间,约一半的成员含有3’和5’非编码区;各成员不均匀地分布于第1,2,3,4,5,6,7和9号染色体上;系统发育关系揭示其成员之间具有较高的遗传多样性。该研究不仅有助于为了解该基因家族的进化起源提供参考,而且可为进一步分析该家族成员的功能奠定基础。
