Aimin Li~1,Jinjin Jiang~2,Yongtai Zhang~1 and Youping Wang~(2*) 1 Jiangsu Institute of Agricultural Science in the Lixiahe District,Yangzhou 225009 2 College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009
None yellow-seed exists in natural B.napus.The objective of this study was to carry out character observation ...
Jinjin Jiang~1,Aimin Li~2,Yongtai Zhang~2 and Youping Wang~(1*) 1 College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009 2 Jiangsu Institute of Agricultural Science in the Lixiahe District,Yangzhou 225009
The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.
Fang KongCailin GeXiaoping FangRod J. SnowdonYouping Wang
Breeders have focused on yellow-seeded Brassica napus （rapeseed） for its better quality com- pared with the black-seeded variety. Moreover, flavonoids have been associated with this kind of rapeseed. In this study, we applied lipid chromatography-electrospray ionization mass spec- trometry （LC-ESI-MSn ） to compare flavonoids in developing seeds of natural black-seeded B. na- pus and yellow-seeded introgression lines selected from progenies of B. napus–Sinapis alba so- matic hybrids. Aside from the most abundant phenolic compounds （sinapine and sinapic acid） and 1, 2-disinapoylglucose, 16 different flavonoids were identified and quantified, including （-）- epicatechin, five monocharged oligomers of （-）-epicatechin （[DP 2]- , [DP 3]- , [DP 4] [DP 2]- B2 and [DP 2]- B5）, quercetin, kaempferol, isorhamnetin-dihexoside, kaempferol-sinapoyl-trihexoside, isorhamnetin- sinapoyl-trihexoside, isorhamnetin-hexoside-sulfate, and isorhamnetin-3-O-glucoside. Most of the flavonoids accumulated with seed development, whereas some rapidly decreased during maturation. The content of these flavonoids was lower in the yellow-seeded materials than in the black seeds. In addition, variations of insoluble procyanidin oligomers and soluble phenolic acids were observed among both rapeseed varieties. Transcriptome changes of genes participating in the flavonoid pathway were discovered by quantitative reverse transcription polymerase chain reaction analysis. Consistent with flavonoid changes identified by high performance liquid chromatography analysis, the expression of most genes in the flavonoid biosynthetic pathway was also downregulated.
Jinjin JiangYanlin ShaoAimin LiChunliang LuYongtai ZhangYouping Wang
