With the disappearance of rigid employment contract and the-intenseness of competition of human resources, turnover has become a great obstacle to firm's well-runned development. From the point of firm's gender composition, the influence of gender composition on gender-based turnover in each level including leading post remains unclear. The paper explores this issue by using statistical methods on the basis of 332 individual sampling data in 65 different enterprises. The results show that proportion of male to female in high and first-line level has significantly positive influence on employee turnover for both male and female. But the proportion of male to female in middle level has significantly negative influence on employee turnover for both male and female. The whole proportion of male to female in organization has positive influence on turnover. The leader's gender has negative effect on turnover of male employee. Most of the above correlations are consistent with foreign researches. Referred to our results, enterprise could control turnover and improve the organization efficiency by adjusting the proportion of male to female in different levels when we make decisions on human resources management in order to attract female employees.