Effects of hypoxia on learning-memory function and swimming capability of rat were studied and the ergonomics under hypoxic condition was also evaluated from the biological point of view.Three modes of hypoxia were designed and plots of oxygen concentration versus time for each group in hypoxic environment were produced.Results showed that the effects of hypoxia on learning-memory function and swimming capability were related with the time and strength of hypoxia.It had nothing to do with the individual difference of rat models.10% O2 long-term intermittent anamorphosis hypoxia could improve the swimming capability of rat model significantly.Stimulating with proper level of hypoxia,carbon dioxide could improve ergonomics in airtight hypoxia environment.Under hypoxia condition,from the ergonomic point of view,6% O2 is the important threshold and might belong in critical region.
This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes.
ZHOU Jin XU JianDong XIE Yao QU Feng DENG YuLin GENG LiNa
