您的位置: 专家智库 > >

国家自然科学基金(51076079)

作品数:23 被引量:152H指数:7
相关作者:杨旭丁书茂杜娟秦龙娟唐佳琦更多>>
相关机构:华中师范大学清华大学咸宁学院更多>>
发文基金:国家自然科学基金国家科技支撑计划国家高技术研究发展计划更多>>
相关领域:环境科学与工程医药卫生生物学理学更多>>

文献类型

  • 23篇中文期刊文章

领域

  • 12篇环境科学与工...
  • 11篇医药卫生
  • 1篇生物学
  • 1篇理学

主题

  • 7篇小鼠
  • 4篇细胞
  • 4篇邻苯二甲酸二...
  • 4篇过敏
  • 4篇过敏性
  • 4篇DEHP
  • 3篇哮喘
  • 3篇甲酸
  • 3篇苯二甲酸
  • 3篇丙二醛
  • 3篇PREVAL...
  • 2篇丁酯
  • 2篇氧化应激
  • 2篇水迷宫
  • 2篇氯氰菊酯
  • 2篇介导
  • 2篇菊酯
  • 2篇健康
  • 2篇高效氯氰菊酯
  • 2篇儿童

机构

  • 17篇华中师范大学
  • 2篇清华大学
  • 2篇咸宁学院
  • 1篇广州医学院
  • 1篇复旦大学
  • 1篇东南大学
  • 1篇哈尔滨工业大...
  • 1篇上海理工大学
  • 1篇西安建筑科技...
  • 1篇天津大学
  • 1篇山西大学
  • 1篇新疆医科大学
  • 1篇重庆大学
  • 1篇中南大学
  • 1篇哥本哈根大学

作者

  • 16篇杨旭
  • 4篇丁书茂
  • 3篇杜娟
  • 3篇尤会会
  • 3篇秦龙娟
  • 3篇唐佳琦
  • 2篇屈芳
  • 2篇马萍
  • 2篇张寅平
  • 2篇武阳
  • 2篇程静菲
  • 2篇柯玉洁
  • 2篇周鄂生
  • 2篇胡传禄
  • 2篇郭靖
  • 2篇尚帅
  • 2篇袁均林
  • 2篇张亚然
  • 2篇廖晓梅
  • 2篇秦晓丹

传媒

  • 5篇生态毒理学报
  • 4篇Scienc...
  • 3篇玉林师范学院...
  • 3篇医学研究杂志
  • 2篇环境科学学报
  • 2篇科学通报
  • 1篇中国环境科学
  • 1篇华中师范大学...
  • 1篇Chines...
  • 1篇公共卫生与预...

年份

  • 1篇2014
  • 7篇2013
  • 6篇2012
  • 7篇2011
  • 2篇2010
23 条 记 录,以下是 1-10
排序方式:
蚂蚁提取液对DNA-蛋白质交联修复能力的研究
2011年
目的探讨蚂蚁提取液(ant extract,AE)对DNA-蛋白质交联(DPC)的修复能力。方法以甲醛为染毒液,预处理小牛胸腺DNA和卵清蛋白混合液,使之形成DPC,再用不同浓度的AE处理,最后采用SDS-KCl沉淀法来检测不同处理组的DPC修复情况。结果当甲醛染毒浓度为0.544mol/L时,10μg/ml的小牛胸腺DNA和10μg/ml的卵清蛋白DNA-蛋白质交联程度最高;制备2%的AE时,选择pH 7.6的磷酸氢二钠-磷酸二氢钠缓冲液作为组织匀浆液,AE具有最强的DPC修复能力,且用pH 3.6的柠檬酸-柠檬酸三钠缓冲液制备的AE也具有部分DPC修复能力;0.002%、0.02%、0.2%和2%的AE具有一定的DPC修复能力,且存在明显的浓度-效应关系。结论本研究确定了蚂蚁提取液制备过程中缓冲液的最佳pH值,并证明了蚂蚁提取液具有一定的DPC修复能力。
廖静周梦晨王娜娜杨旭
关键词:蚂蚁DNA-蛋白质交联
邻苯二甲酸二乙基己酯(DEHP)对小鼠的氧化损伤及黄豆异黄酮对其保护作用的研究(英文)被引量:4
2012年
邻苯二甲酸二乙基己酯(DEHP)是环境中广泛存在的污染物,作为一种内分泌干扰物,已严重威胁到人类健康和生态环境安全。每天用250、500和1000mg·kg-1·d-1的DEHP对雄性昆明小鼠进行灌胃处理,连续处理30d后,分别考察了小鼠的器官相对质量、丙二醛(MDA)和谷胱甘肽(GSH)的含量以及睾丸组织学等指标的变化。结果显示,小鼠的肝、肺组织肿大,睾丸严重萎缩,并呈剂量依赖性。肝脏MDA含量显著增加,肝脏和血液GSH水平显著下降。从睾丸组织学分析结果可见,精子生长发育条件已受到破坏,例如睾丸细胞坏死和凋亡,生精小管上皮细胞大量脱落等。DEHP对未成熟的雄性小鼠有氧化损伤作用和生殖毒性,特别是在较高剂量(500and1000mg·kg-1·d-1)时。为了探讨大豆异黄酮(SI)对DEHP诱导的氧化损伤和睾丸损伤的保护作用,将暴露于1000mg·kg-1·d-1DEHP的小鼠,用100mg·kg-1·d-1剂量的SI平行处理。结果显示,SI作为抗氧化剂和雌激素调节剂可明显减弱DEHP所致的氧化应激和睾丸毒性。
王莉郭靖韩冰秦龙娟杨旭
关键词:睾丸损伤抗氧化剂雌激素调节
农药高效氯氰菊酯对小鼠肾细胞氧化损伤的研究被引量:5
2011年
为了研究农药高效氯氰菊酯对生物体的氧化损伤,以昆明小鼠为受试体,高效氯氰菊酯按10、20和40mg/kg 3个剂量水平,灌胃染毒小鼠7d,以肾匀浆测定ROS和GSH,以肾细胞测定DPC系数.实验结果表明:随着高效氯氰菊酯染毒剂量的升高,ROS和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量≥20mg/kg时,ROS和DPC系数有显著差异(p<0.05),GSH有极显著差异(p<0.01),说明较高剂量的高效氯氰菊酯可造成小鼠肾脏的氧化损伤.
马萍丁书茂秦龙娟张亚然杜娟杨旭
关键词:高效氯氰菊酯小鼠
邻苯二甲酸二丁酯(DBP)与卵清白蛋白(OVA)联合染毒对小鼠肺脏和脾脏组织的氧化应激被引量:1
2013年
为研究邻苯二甲酸二丁酯(dibutylphthalate,DBP)单独染毒及与卵清白蛋白(ovalbumin,OVA)联合染毒对小鼠肺脏和脾脏组织氧化应激的作用,将BALB/c小鼠随机分为8组:(1)未处理对照组(生理盐水组);(2)0.5mg·kg-1DBP染毒组;(3)5.0mg·kg-1DBP染毒组;(4)50mg·kg-1DBP染毒组;(5)1.67mg·kg-1OVA单独染毒组;(6)0.5mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组;(7)5.0mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组;(8)50mg·kg-1DBP与1.67mg·kg-1OVA联合染毒组。未处理对照组和DBP染毒组每天按体质量给予生理盐水和DBP灌胃。2周后,测定肺脏组织活性氧物种(ROS)、谷胱甘肽(GSH)和丙二醛(MDA)含量以及脾脏组织ROS、GSH含量。结果显示,联合染毒组相较于其他组的肺脏组织各指标均有不同的显著性差异(P<0.05),联合染毒组的脾脏组织中ROS含量较其他组有显著差异(P<0.05),而GSH含量无统计学意义(P>0.05)。结果说明,DBP与OVA联合染毒能够增强肺脏组织的氧化应激作用,对于脾脏组织的氧化应激作用不明显;DBP在联合染毒中显示一定免疫佐剂效应。
陈绍恢尤会会毛琳问华肖叶染枫杨旭
关键词:邻苯二甲酸二丁酯小鼠氧化应激
高效氯氰菊酯对小鼠肝细胞的氧化损伤被引量:17
2012年
为了探讨高效氯氰菊酯对生物体的氧化损伤,以昆明小鼠为受试体,高效氯氰菊酯按10、和40mg·kg20-13个剂量水平,灌胃染毒小鼠7d,并以肝匀浆测定活性氧自由基(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)含量,以肝细胞测定DNA-蛋白质交联(DPC)系数.实验结果表明,随着高效氯氰菊酯染毒剂量的升高,ROS和MDA含量及DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量≥20mg·kg-1时,处理组的ROS含量和DPC系数与对照组有显著差异(p<0.05);染毒剂量≥40mg·kg-1时,GSH和MDA含量与对照组有显著差异(p<0.05),DPC系数有极显著差异(p<0.01).说明较高剂量的高效氯氰菊酯可造成小鼠肝脏的氧化损伤和DNA-蛋白质交联作用增强.
马萍秦龙娟张亚然杜娟尤会会杨旭
关键词:高效氯氰菊酯小鼠DNA损伤
DEHP暴露对小鼠神经行为学及脑脂质过氧化物的影响被引量:11
2012年
为研究增塑剂邻苯二甲酸二乙基己酯(Di-2-ethylhexyl phthalate,DEHP)对脑组织造成氧化损伤以及对小鼠神经行为(主要包括学习、记忆能力与情绪)的影响.将24只雄性昆明小鼠随机分成4组,每组6只,分别为对照组(生理盐水),低浓度染毒组(5mg·kg-1DEHP,L组),中浓度染毒组(50mg·kg-1DEHP,M组),高浓度染毒组(500mg·kg-1DEHP,H组).利用Y型电迷宫检验小鼠的学习记忆能力变化,利用悬尾实验和强迫游泳实验检测小鼠抑郁行为的变化.然后取出脑组织,检验活性氧簇(ROS)及丙二醛(MDA)含量.数据显示,染毒组小鼠的学习记忆及行为学控制能力明显低于对照组(p<0.05),并且随DEHP浓度的增大小鼠的学习记忆及行为学控制能力降低.DEHP低、中、高剂量染毒组与对照组氧化应激反应均具有显著性(p<0.05),且呈剂量效应关系.因此,DEHP可导致小鼠学习记忆能力下降、抑郁情绪增加,其分子机制可能涉及DEHP对脑组织造成的氧化损伤.
周佩蒋子奇钏茂巧程静菲尚帅唐佳琦杨旭
关键词:邻苯二甲酸二乙基己酯小鼠
Ten cities cross-sectional questionnaire survey of children asthma and other allergies in China被引量:22
2013年
Asthma,rhinitis and eczema(allergic or non-allergic)have increased throughout the world during the last decades,especially among children.Changes in the indoor environment are suspected to be important causes.China has experienced a dramatic change in indoor environmental exposures during the past two decades.However,such changes and their associations with children’s asthma and other health aspects have not been thoroughly studied.China,Children,Homes,Health(CCHH),Phase I,was a cross-sectional questionnaire survey of 48219 children 1–8 years old in 10 Chinese cities during 2010–2012.The questionnaire includes the International Study of Asthma and Allergies in Childhood(ISAAC)core health questions and additional questions regarding housing,life habits and outdoor environment.In health analyses,children aged 3–6 years old were included.The prevalences of doctor diagnosed asthma varied from 1.7%to 9.8%(mean 6.8%),a large increase from 0.91%in 1999 and 1.50%in2000.The prevalence of wheeze,rhinitis and atopic eczema(last 12 months)varied from 13.9%to 23.7%,24.0%to 50.8%and4.8%to 15.8%,respectively.Taiyuan had the lowest prevalences of all illnesses and Shanghai the highest,except for wheezewhere the highest value was for Urumqi.We found(1)no obvious association between disease prevalences and ambient PM10concentrations and(2)higher prevalences of disease in humid climates with hot summers and cold winters,but with no centrally heated buildings.Associations between the diseases and economic status as indexed by Gross Domestic Product(GDP)requires further study.
ZHANG YinPingLI BaiZhanHUANG ChenYANG XuQIAN HuaDENG QiHongZHAO ZhuoHuiLI AnGuiZHAO JiaNingZHANG XinQU FangHU YuYANG QinWANG JuanZHANG MingWANG FangZHENG XiaoHongLU ChanLIU ZhiJianSUN YueXiaMO JinHanZHAO YiLiLIU WeiWANG TingTingNORBCK DanBORNEHAG Carl-GustafSUNDELL Jan
关键词:过敏性
Increasing prevalence of asthma and allergy in Beijing pre-school children: Is exclusive breastfeeding for more than 6 months protective?被引量:3
2013年
This study evaluated the prevalence and risk factors for asthma,allergy and related symptoms;and breastfeeding patterns and durations for 5479 Beijing children aged 3–6.Parents of children in randomly selected kindergartens wrote responses to a questionnaire used previously.The study aimed to evaluate trends in the prevalence of asthma and related illnesses,and to determine whether"more"breastfeeding,defined as exclusive,>6 months,was associated with reduced prevalence.Asthma has increased in this age group between 1990 and 2011,with the steepest increase in the last 2–3 years.Of the total,14.2%(779)children were breastfed exclusively for>6 months.The efficacy of"more"breastfeeding was tested in a subset with two strong risk factors,positive family history(for asthma and/or allergy)and male gender."More"breastfeeding was found to be significantly protective(aOR 0.42,P<0.05)for this subset against Doctor-diagnosed asthma(D-asthma).Protection that did not reach statistical significance was also found for this subset against Wheeze ever,Cough at night,Rhinitis ever,Doctor-diagnosed rhinitis(D-rhinitis)and Eczema.The greatest protective effects were found for girls with no family history of asthma or allergy,reaching statistical significance for Wheeze ever(aOR 0.48,P<0.01),Cough at night(aOR 0.47,P<0.01),D-asthma(aOR 0.14,P<0.01)and Rhinitis ever(aOR 0.67,P<0.05)."More"breastfeeding was not consistently associated with either a protective or risk effect for Eczema.
QU FangWESCHLER Louise B.SUNDELL JanZHANG YinPing
Effects of home environment and lifestyles on prevalence of atopic eczema among children in Wuhan area of China被引量:3
2013年
Atopic eczema is a common health problem in children.The prevalence has increased in the past decades.Besides a genetic predisposition,lifestyle factors,dietary habits and indoor environments are thought to be related to its high prevalence.A cross-sectional questionnaire study was carried out in 2011 in the Wuhan area to study associations between home environments and children’s health.The questionnaires were replied to by parents of 2193 children 1 8 years old and the response rate was91.4%(2193/2400).The results show:(1)The prevalence of"eczema symptom ever that lasted for 6 months"was 23.2%;"eczema symptom in the last 12 months"7.4%and"awake at night due to itchy rash"3.4%.(2)Risk factors:Closer to business area(AOR 1.4),moisture related problems(AOR 1.3 2.2),moldy and stuffy odor perception(AOR 1.5),mice observed(AOR 1.8),pet keeping(AOR 1.3),exposure to environmental tobacco smoke(AOR 1.3)and incense(AOR 1.8)were significant risk factors for atopic eczema symptoms ever.Living in an apartment,compared to single family house,was related to more eczema symptom in the last 12 months.Damp clothing/bed sheets and perception of stuffy odor were risk factors for both"eczema symptom in the last 12 months"and"awake due to itchy rash(severe eczema)".The odds ratio of mice observed for severe eczema was 3.1(95%CI:1.4 6.7).It suggests that urbanization,life style and dampness problems at home were significant risk factors for eczema symptoms among children in Wuhan,China.
ZHANG MingWU YangYUAN YeZHOU EShengSUNDELL JanSUN YueXiaYANG Xu
关键词:儿童健康家庭环境过敏性
Indoor environmental quality and the prevalence of childhood asthma and rhinitis in Wuhan area of China被引量:4
2013年
Indoor environmental quality is suspected to be at least part of the cause of the increasing prevalence of childhood asthma and allergy.This study is part of the China,Children,Homes,Health(CCHH)project,which was designed to identify the risk factors indoors that are associated with prevalence of asthma and allergy among children in China.A cross-sectional questionnaire study was carried out in Wuhan in 2011.The questionnaire was a modified(to Chinese building characteristics)version of a questionnaire used first in Sweden.The parents of 2193 children,aged 1–8 years,replied,a response rate of 91.4%(2193/2400).Prevalences of asthma and allergic symptoms were calculated,and a Chi-square test and multiple logistic regression were used to identify risk factors.Prevalences of health outcomes are"wheezing last 12 months"18.5%,"cough at night last 12 months"15.4%,"doctor-diagnosed asthma"6.0%,"rhinitis last 12 months"48.7%,"doctor-diagnosed allergic rhinitis"17.5%.Factors associated with increased risk for asthma,allergy and related symptoms include living in an urban area,dampness(significant),use of gas for cooking,new dwelling decoration(paint and furniture obtained during pregnancy),keeping pets and breast feeding less than 3months.Factors associated with reduced risk as installing an exhaust fan in the bathroom.Home environmental factors are significantly associated with the prevalence of childhood asthma and rhinitis in Wuhan.Urbanization and dampness problems at home are significant risk factors for doctor diagnosed asthma and allergic rhinitis among children in Wuhan.
ZHANG MingZHOU EShengYE XinSUN YueXiaSUNDELL JanYANG Xu
关键词:室内环境质量过敏性鼻炎LOGISTIC回归分析
共3页<123>
聚类工具0