Zostera marina, a monocotyledonous angiosperm, is one of the most important seagrass species. To inves- tigate the salt-tolerance mechanism and discover salt-tolerant genes in Z. marina, a cDNA library was con- structed. Single-pass sequencing of the 5＇ ends of 4 081 clones yielded 4 002 high quality expressed sequence tags （ESTs）, which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from 100-1 500 bp. Basic Local Alignment Search Tool （BLASTX） analysis revealed that 1 664 unigenes had significant homology to known genes in the Na- tional Center for Biotechnology Information （NCBI） non-redundant （nr） database （E-value≤5-10）. Among them, the two most abundant genes encoded metallothionein （157 ESTs） and chlorophyll a/b-binding pro- tein （38 ESTs）, accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes （KEGG）, 1 462 unigenes were assigned to 1 161 pathways （E-value≤5-10）. A total of 938 unigenes were assigned Gene Ontology （GO） terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes for metallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z. marina.
KONG FannaZHOU YangSUN PeipeiLIU LiminMAO Yunxiang
A highly reliable interface of self-aligned barrier CuSiN thin layer between the Cu film and the nano-porous SiC：H （p-SiC：H） capping barrier （k=3.3） has been developed in the present work. With the introduction of self-aligned barrier （SAB） CuSiN between a Cu film and a p-SiC：H capping barrier, the interfacial thermal stability and the adhesion of the Cu/p-SiC：H film are considerably enhanced. A significant improvement of adhesion strength and thermal stability of Cu/p-SiC：H/SiOC：H film stack has been achieved by optimizing the pre-clean step before caplayer deposition and by forming the CuSiN-like phase. This cap layer on the surface of the Cu can provide a more cohesive interface and effectively suppress Cu atom migration as well.
The present study has theoretically investigated the combined torsional buckling of double-walled carbon nanotubes (DWCNTs) with axial load in the multi-field coupled condition. The effects of torsion, axial load, thermal-electrical change, surrounding elastic medium and the Van der Waals forces are all taken into consideration. The governing equation of buckling for CNTs subjected to thermo-electro-mechanical loadings has been established based on an elastic shell model of continuum mechanics. Reasonable simplifications are made to get the explicit expression of the critical buckling shear stress of DWCNTs, and numerical experiments are conducted for further research. It is shown that under certain electric and temperature field the critical buckling shear stress of DWCNTs only depends on the wave number of buckling modes. On the other hand, all the related impact factors have enormous influence on the critical buckling shear stress under a certain buckling mode. The critical buckling shear stress changes linearly with the axial-to-shear stress ratio, as well as the thermal and electric change. Axial compression tends to make DWCNTs unstable, while axial tension benefits the buckling stability. The critical buckling shear stress is directly proportional to the applied voltage. At room or lower temperature, the critical shear stress for infinitesimal buckling increases as the temperature change increases, while it decreases at a higher temperature. The conclusions are useful for the design of nano-structures related to the buckling stability of DWCNTs.
