Cellulase activities of Anoplophora glabripennis （Motsch.） adults from two host plants （Populus simonii x p pyramidliscr cv. Opera Hsu. and Salix matsudana Koidz） fed on three different host tree species （Acer negundo Linn., S. matsudana Koidz and P simonii x P pyramidliscr cv. Opera Hsu.） were investigated. Enzyme activities of endoglucanase and β-glucosidase in the intestines of the insects were measured. The results show that there are no statistically significant differences in the enzyme activities of endoglucanase between male and female insects from the two host plants fed on three host trees, neither inβ-glucosidase. No statistically significant differences in the enzyme activities of endoglucanase and β-glucosidase were found between males and females from the two plants fed on three host tree species, except that the endoglucanase activity in males from the opera poplar fed on Hankow willow is lower than that fed on ash-leaf maple and opera poplar.
LI Xiao-juanYAN Xiong-feiLUO You-qingTIAN Gui-fangNIAN Yin-jieSUN Hong
The Asian longhorned beetle （ALB）, Anoplophora glabripennis （Motschulsky） is a polyphagous woodborer of hardwood trees. In order to well understand the oviposition preference of A. glabripennis emerging from different larval host tree species, we selected five common host tree species in the field and evaluated its oviposition preferences. The five host tree species are Acer negundo （AN）, Salix matsudana f. lobato-glandulosa （SM）, Populus nigra L. var. thevestina （Dode） Bean （PN）, P. simonii × P. pyramidalis cv. Opera 8277 （PS） and P. alba var. pyramidalis （PA）. Results show that adult beetles emerging from these five tree species almost have the same oviposition phenomenon. All adult beetles, except those emerging from PS, chewed the highest potential sites and laid most eggs on AN trees. Adult beetles emerging from AN, PN, PS and PA did not chew any sites nor laid any eggs on PA trees. These results showed that adult A. glabripennis emerging from the five host tree species did not show any clear oviposition preference for their larval host tree species in the field but they did present the most obvious preference for AN and the lowest preference for PA.
YAN Xiong-feiLI Xiao-juanLUO You-qingXU Zhi-chunTIAN Gui-fangZHANG Tie-lin
In order to investigate different kinds of cellulase in insect pests, we selected male and female adults of Anoplophora glabripennis （Motschulsky） emerging from four different host species （Populus alba var. pyramidalis, P. nigra var. thevestina （Dode） Bean., P. simonii × P. pyramidliscr cv. Opera 8277 Hsu. and Salix matsudana f. lobato-glandulosa） as our research material. The enzyme activitives of three kinds of cellulase in the intestines of the adult insects were measured. The Cx-cellulase isozymes were detected with a CMC-incorporated polyacrylamide gel. The results show that： there are no statistically significant differences between the enzyme activities of males and females emerging from the different host species. The order of magnitude in activity is： Cx-cellulase 〉 β-glycosidase 〉 C1-cellulase. When the adults emerge from the same host species, there are no statistically significant differences between male and female enzyme activities of β-glycosidase and C1-cellulase, but the enzyme activity of Cx-cellulase of males is clearly higher than that of females. The patterns and migration of Cx-cellulase isozymes of males and females emerging from differ-ent hosts trees are clearly not different, and neither are they different when emerging from the same hosts.
LI Xiao-juanYAN Xiong-feiLUO You-qingTIAN Gui-fangSUN Hong
