Objectives: To compare the efficiency of four commer-cial ELISA kits in detecting type-specific HSV-2 IgGantibodies.Materials and Methods: A total of 125 subjects, includ-ing 105 with genital ulcers, and 20 controls without anyhistory of STDs were recruited from the STD clinic fordetection of type-specific HSV-2 IgG antibody with dif-ferent kits. Four kinds of commercially available ELISAkits, including Quida HSV2 IgG ELISA (AifulangBiochem Co. Ltd., Hangzhou), TORCH-HSV2 IgG(Jingmei Biotech Co. Ltd., Shanghai), CaptiaTM HSV2 IgG(Trinity biotech, USA ) and HerpeSelect TM 2 ELISA IgG(Focus technologies, USA) were used for evaluation. West-ern Blot assay was performed as a gold standard.Results: Compared to Western Blot results, the sensi-tivity and specificity of the kits (Quida HSV2, TORCH-HSV2, CaptiaTM HSV2 and HerpeSelectTM 2) were 13.1% and 98.4%, 7.5% and 100%, 100% and 11.1%,87.7% and 96.7%, respectively. The positive predictivevalue (PV) and negative PV of the four kits were 88.9%and 54.3%, 100% and 55.5%, 55.6% and 100%, 96.2%and 89.2%, respectively. The areas under the ROC curveof three kits (Quida HSV2, CaptiaTM HSV2 andHerpeSelectTM 2) were 0.885 (0.822～ 0.948), 0.825 (0.747- 0.902), 0.974 (0.950- 0.998), respectivelyConclusion: The performance of HerpeSelectTM 2 is thebest among the four kits. The results also indicate thatthe commercially available kits for detection of type-spe-cific HSV2 antibody should be re-evaluated in terms oftheir validity.
