The resistance gene Pi36 confers a stable and high level of resistance against Chinese isolates of the rice blast pathogen Mag-naporthe oryzae.Quantitative real time RT-PCR was used to investigate the expression profiles of various resistance-related genes in both the Pi36/AvPi36 incompatible interactions.Among 17 members of the OsMAPK family,OsMAPK2,OsMAPK4,OsMAPK8 and OsMAPK15 were differentially expressed,as were OsWRKY30,OsWRKY32,OsWRKY64 and OsWRKY67 among the 13 OsWRKY members studied.The induction of the expression of genes from both of these families suggests that both the JA and SA pathways are involved in Pi36-mediated defence.Other genes differentially expressed included the OsbZIP transcription factors,OsNIF1 and OsNIF2,and the NPR1 homologue OsNH2,all of which were reported in the rice blast pathosystem.The in-volvement of these genes illustrates the complexity of the downstream signalling pathways involved in the Pi36/AvrPi36 interaction.
LIU XinQiongLI YuanYuanWANG LiYuanLIU XueQunWANG ChunTaiWANG LingPAN QingHua
Objective The aim was to explore conditions of genetic transformation for Indica rice Kasalath and laid a foundation for further study on molecular biology. Method With callus of Kasalath as transformation receptor, Agrobacterium tumefaciens-mediated method was used to conduct genetic transformation. The genetic transformation system was optimized from several aspects, including co-culture mode, co-culture time and the affertreatment method of co-culture. Result The results showed that two days is the best co-culture time for genetic transformation, the acquisition rate of resistant callus was up to 84.1%, and transformation rate was up to 73%. Whether callus contact to the culture medium directly or indirectly has no significant effect on transformation. [ Conclusion] Genetic transformation successfully transferred exogenous gene OsMAPk2 into the rice genome.
