In this study, we simulated and analyzed the monthly variations of stable water isotopes in different reservoirs at Manaus, Brazil, using the Community Land Model (CLM) that incorporates stable isotopic effects as a diagnostic tool for understanding stable water isotopic processes, filling the observational data gaps and predicting hydrometeorological processes. The simulation results show that the δ 18O values in precipitation, vapor and surface runoff have distinct seasonality with the marked negative correlations with corresponding water amount. Compared with the survey results by the International Atomic Energy Agency (IAEA) in co-operation with the World Meteorological Organization (WMO), the simulations by CLM reveal the similar temporal distributions of the δ 18O in precipitation. Moreover, the simulated amount effect between monthly δ 18O and monthly precipitation amount, and MWL (meteoric water line) are all close to the measured values. However, the simulated seasonal difference in the δ 18O in precipitation is distinctly smaller than observed one, and the simulated temporal distribution of the δ 18O in precipitation displays the ideal bimodal seasonality rather than the observed single one. These mismatches are possibly related to the simulation capacity and the veracity in forcing data.