The mixture compound extracted by supercritical carbon dioxide extraction can attracted the insect directly,which was the new approach to search after the attractant. Volatiles could be used to bioassay. Results of behavior responses of Anoplophora glabripennis to the volatiles extracted from Acer mono under different pressure and temperature showed that extracts of 25 MPa, 35 ℃ was attractive to A. glabripennis significantly than others. Extracts of 25 MPa, 35 ℃ was the optimum supercritical fluid extraction(SFE) condition. The analysis of GC-MS showed that the main compound were 3-hexenol and ethyl acetate.
The volatile compositions of Chinese Pine (Pinus tabulaeformis) are analyzed. The main turpentine volatiles are (R)-(+)-α-pinene, (S)-(-)-β-pinene, (S)-(+)-3-carene (the largest) which occupy more than 97% of the total. There are also myrcene, (+)-limonene, camphene, terpinen and phellandrene. In the experiment of Electroantennograms(EAG), the male of red turpentine beetles are sensitive to (S)-(-)-α-pinene、limonene and (S)-(+)-3-carene; while the female of the red turpentine beetles are sensitive to (S)-(+)-3-carene、limonene、(S)-(-)-α-pinene and myrcene. The female are more sensitive to the volatiles than the male. There is much difference in response of Dendroctonus valens in Chinese pine compared to that in USA in ponderosa pine. The volatile difference of the two pine species and the environment condition may contribute to this change. Further study on the mechanism of the change is needed.
