Objective:To investigate the impact of the preinduced intestinal heat shock protein 70(HSP70)on the visceral hypersensitivity and abnormal intestinal motility in a post-infectious irritable bowel syndrome(PI-IBS) mouse model.Methods:Eighty-four female C57BL/6 mice were randomly assigned to four groups:control group(n=21) and induction+PI-IBS group(n=21),PI-IBS group(n=21) and induction group(n=21).The mice in PI-IBS group were infected in vivo with trichinella spiralis by oral administration.The visceral hypersensitivity and intestinal motility were evaluated respectively with abdominal withdrawal reflex and colon transportation test.The intestinal HSP70 protein and mRNA level was measured by Western blot and realtime PCR.Meanwhile,the intestinal proinflammatory cytokines IL-10 and TNF-α level was detected by ELISA.Results:Compared with their counterparts in PI-IBS group,the animals in the Induction+PI-IBS group show significantly increased intestinal level of HSP70 and obviously ameliorative clinical tigurcs.including abdominal withdrawal reflex score,intestine transportation time and Bristol scores(P<0.05).Meanwhile,the intestinal post-inflammatory cytokines remarkably changed,including increased IL-10 level and decreased TNF-αlevel(P<0.05).Conclusions:Intestinal IISP70 may play a potential protective role through improving the imbalance between the intestinal post-inflammatory and anti-inflammatory cytokines in PI-IBS.
Cheng LanXiao-Ning SunXu-Chun ZhouBo YangBai-Li HuangTao-Zhi DengZhou-Tao HeXiang-Yang Han
目的探讨内镜下金属钛夹联合兰索拉唑治疗对老年上消化道出血患者凝血功能的影响。方法选取2014年6月至2019年6月海南省人民医院老年上消化道出血患者102例,随机分为对照组(n=51)和观察组(n=51)。对照组给予兰索拉唑治疗,观察组在其基础上给予内镜下金属钛夹联合治疗,治疗后3 d对效果进行评估,比较两组止血效果、纤维蛋白原(FIB)、凝血活酶时间(APTT)、凝血酶原时间(PT)、血小板(PLT)水平、血清内毒素(LPS)、C反应蛋白(CRP)水平、并发症发生率。结果观察组即时止血、72 h止血率均高于对照组(P<0.05);观察组与对照组治疗过程中并发症发生率无统计学意义(P>0.05);观察组治疗后3 d凝血功能PT、APTT时间长于对照组(P<0.05);FIB、PLT水平低于对照组(P<0.05);两组治疗后3 d LPS、CRP水平低于治疗前(P<0.05);观察组治疗后3 d LPS、CRP水平低于对照组(P<0.05)。结论内镜下金属钛夹联合兰索拉唑用于老年上消化道出血中能获得良好的止血效果,有助于改善凝血功能,未增加并发症发生率。
