Pine wilt disease,caused by Bursaphelenchus xylophilus and Bursaphelenchus mucnatus,is a serious quarantined disease.Arboreal nematode-trapping fungi of P inus spp.are effective predators on nematodes and have strong host adaptability.The development of these fungal resources may be an effective way to control pine wood nematodes.We collected 515 samples of pine wilt disease from the areas of Ninghai City(Zhejiang province),Shuangbai County(Yunnan province),and Daxing'anling(Heilongjiang province),China.Through isolation,culture and identification,6 species of nematode-trapping fungi(A rthrobotrys cladodesr,A.oligospora,A.musiformis,A.dendroides,Dactylellina ellipsospora,Monacrosporium thaumasium)were identified for predation against B.xylophilus,and 9 species(Arthrobotrys dactyloides,A.cladodes r A.oligospora A.dendroides,Dactylellina ellipsospora,Dactylella asthenopaga,D.leptospora,Arthrobotrys superba,Monacrosporium drechseri)were identified for predation against B.mucnatus.This study provides information in the classification of arboreal predator nematodes and provides an important basis for future biological control of pine wood nematodes.
Ophiostoma Syd.&P.Syd.(蛇口壳属)建于1919年(见Kirk et al.2001),该属真菌是经济上重要的病原真菌。它的许多种都能引起木材变色,如O.piliferum(Fr.)Syd.&P.Syd.和O.ips(Rumbold)Nannf.是较普遍的木材变色真菌(Verral 1939)。另外,O.ulmi(Buisman)Nannf.是荷兰榆树病的病原菌,致病性强,在欧洲许多地方导致榆树大面积死亡。