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大麦黄矮病毒GAV基因组全序列测定及其结构分析被引量:9
2003年
测定了在中国分离得到由麦二叉蚜和麦长管蚜传播的大麦黄矮病毒GAV的基因组核苷酸全序列,该病毒分离物的RNA由5685个核苷酸组成,内含6个开放阅读框架(ORF)和4个非编码区(UTR),基因组大小和结构与黄症病毒属(Luteovirus)的大麦黄矮病毒PAV(BYDV-PAV)和MAV(BYDV-MAV)相似,序列分析表明,它与BYDV-MAV的PS1分离物基因组序列的同源性最高。在6个开放阅读框架中,除ORF6核苷酸序列同源性为72.0%外,其他ORF的核苷酸序列同源性均大于90%,两者全基因组的同源性为90.4%,推导的编码产物氨基酸序列同源性除P6和通读蛋白(RTP)分别为67.4%和87.4%外,其他均大于90%,其中外壳蛋白(CP)为95.5%,根据与BYDV-MAV的相似性,BYDV-GAV应是一种与BYDV-MAV类似的病毒。
晋治波王锡锋常胜军周广和
关键词:基因组结构分类学基因序列测定核苷酸同源性
The complete nucleotide sequence and its organization of the genome of Barley yellow dwarf virus-GAV被引量:10
2004年
The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV genome were similar to those of BYDV PAV-aus. The nucleotide and deduced amino acid sequences of the six ORFs were aligned and compared with those of other luteoviruses. The results showed that there was a high degree of identity between BYDV-GAV and MAV-PS1 in all ORFs except ORF5 and ORF6, which had only 87.4% and 70.2% identities respectively. The reported genomic nucleotide sequence of MAV was shorter than that of BYDV-GAV, but the comparison of the genomic nucleotide sequences for MAV-PS1 and GAV showed 90.4% sequence identity for the same region of the genome. Ac-cording to the level of sequence similarities, BYDV-GAV should be closely related to BYDV-MAV.
JIN Zhibo WANG Xifeng CHANG Shengjun ZHOU Guanghe
关键词:BARLEYYELLOWDWARFGENOMIC
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