羊的梨形虫病在世界范围内广泛分布,由多种泰勒虫和巴贝斯虫引起。在我国主要有莫氏巴贝斯虫、羊巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫,给我国畜牧业造成了巨大的经济损失。为了快速准确地鉴定羊梨形虫,作者运用PCR技术扩增了梨形虫的18 S rRNA基因,然后再分别用内切酶ClaⅠ、Sau96Ⅰ和MboⅡ酶切扩增产物,根据酶切后限制性片段多态性的不同,建立了PCR-RFLP方法,达到了鉴定莫氏巴贝斯虫、巴贝斯虫未定种、吕氏泰勒虫、尤氏泰勒虫和绵羊泰勒虫的目的,为梨形虫的分类和流行病学调查提供了新的方法。
Theileria annulata,the causative agents of tropical theileriosis,is protozoan parasites that cause lymphoproli...
Youquan Li~(1-6),Guiquan Guan~(1-6),Zhijie Liu~(1-6),Jifei Yang~(1-6),Qingli Niu~(1-6),Ze Chen~(1-6),Jianxun Luo, Hong Yin~(1-6*). 1 State Key Laboratory of Veterinary Etiological Biology 2 Key Laboratory of Veterinary Parasitology of Gansu Province 3 Engineering Research Center of Biological Detection of Gansu Province 4 Key Laboratory of Epizootic Diseases of Grazing Animal of Ministry of Agriculture 5 Key Laboratory of Veterinary Public Health of Ministry of Agriculture 6 Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Science,Xujiaping 1, Lanzhou,Gansu,730046,P.R.China
