目的探讨葛根素对急性视网膜缺血再灌注兔眼模型视网膜组织中游离谷氨酸(gluta-mate)浓度的影响。方法新西兰白兔20只随机分为对照组和实验组,各组右眼应用前房灌注加压法使前房内压力升高至120 mm Hg维持60分钟后再灌注,建立视网膜缺血再灌注模型,左眼为空白对照眼。实验组在前房灌注前2小时视网膜组织腔内注射葛根素溶液,对照组给予等量生理盐水视网膜组织腔内注射。再灌注24小时摘除眼球,采集视网膜组织,研磨及蛋白沉淀后用日立L-8800氨基酸自动分析仪检测游离氨基酸浓度。结果对照组兔右眼视网膜组织中游离谷氨酸浓度较左眼升高,差异有显著性意义(t=9.518,P<0.001)。实验组兔右眼视网膜组织中游离谷氨酸浓度较左眼升高,差异有显著性意义(t=2.519,P<0.05);但较对照组浓度低,差异有显著性(t=6.575,P<0.05)。结论兔视网膜缺血再灌注可致视网膜组织中游离谷氨酸浓度升高,是造成视网膜组织损伤的可能机制之一;而葛根素可以通过阻止谷氨酸浓度升高,从而保护了视网膜组织。
Retinal ischemia-reperfusion injury(RIRI)is a common cause of visual impairment and blindness.At the cellular level,ischemic and reperfuion retinal injury consists of a self-reinforcing destructive cascade involving oxidative stress initiated by energy failure,inflammatory reaction,calcium influx,increased glutamatergic stimulation and neuronal depolarisation and apoptosis.A number of animal models and analytical techniques have been used to study the retinal ischemia-reperfusion injury,we now understand much better than ever before in the mechanism of RIRI,and an increase in the therapeutic strategies has been developed experimentally to attenuate the detrimental effects of retinal ischemia-reperfusion injury.Thus far,however,success in the laboratory has not been translated to the clinic.Given the increasing understanding of the events involved in ischemia-reperfusion neuronal injury,it is hoped that clinically effective treatments for retinal ischemia-reperfusion injury will soon be available.
