A simple spectrophotometric assay of H2O2and glucose using Ag nanoparticles has been carried out.Relying on the synergistic effect of H2O2reduction and ultraviolet(UV)irradiation,Ag nanoparticles with enhanced absorption signals were synthesized.H2O2served as a reducing agent in the Ag nanoparticles formation in which Ag+was reduced to Ag0by O2àgenerated via the decomposition of H2O2in alkaline media.On the other hand,photoreduction of Ag+to Ag0under UV irradiations also contributed to the nanoparticles formation.The synthesized nanoparticles were characterized by TEM,XPS,and XRD.The proposed method could determine H2O2with concentrations ranging from 5.0 10à7to 6.0 10à5mol/L.The detection limit was estimated to be 2.0 10à7mol/L.Since the conversion of glucose to gluconic acid catalyzed by glucose oxidase was companied with the formation of H2O2,the sensing protocol has been successfully utilized for the determination of glucose in human blood samples.The results were in good agreement with those determined by a local hospital.This colorimetric sensor thus holds great promises in clinical applications.
Yu ZhangYan-Jun ZhangXiao-Dong XiaXiao-Qi HouCheng-Ting FengJian-Xiu WangLiu Deng
