Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes theconversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, thepartial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics.Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms (SNPs), one of whichin intron 10 with differing bases (G981A ) is within the restriction site for enzyme Msp Ⅰ. Distribution of Msp Ⅰ -RFLPgenotype and allele frequencies among different pig breeds were studied. By association analysis between Msp Ⅰ PCR-RFLP polymorphism (AA, AB, BB genotypes of HK2 gene intron 10) and some meat quality and carcass traits in F2 group,which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between ABand BB genotypes (P < 0 05) in F2 group. In addition, the pattern of expression of HK2 in a variety of tissues in pig was alsodetermined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle.
WANG Jun DENG Chang-yan XIONG Yuan-zhu ZUO Bo CHENG Huan-chen LI Feng-e LEI Ming-gang ZHENG Rong JIANG Si-wen
