通过电喷雾质谱(ESI/MS)和高效液相色谱-蒸发光散射器(HPLC-ELSD)鉴定和分析用柱层析法纯化大豆卵磷脂得到的各组分主要成分。经质谱检测纯化的组分中主要有两种分子类型的神经酰胺,分别是C18:0 d18:1Cer和C25:0 h d18:1Cer;主要有两种分子类型的脑苷脂,分别是C16:0 t18:2Ce和C17:0Ce。通过HPLC-ELSD分析,二次柱层析得到的神经酰胺含量99.43%,回收率为98.23%;脑苷脂含量为98.86%,回收率为97.27%。
The structured lipids are produced through sn-l,3-specific interesterification of soybean oil with medium-chain triacylglycerol （MCT） in continuous reactions catalyzed by Thermomyces lanuginose lipase （Lipozyme TL IM）. Cheap Lipozyme TL IM presents similar interesterification degree （ID）, sn-l,3-specificity and residual activity as expensive Rhizomucor miehei in batch reactions. In packed-bed interesterification of soybean oil with Mcr cat- alyzed by Lipozyme TL IM, the residence time has a significant effect on ID, while temperature has a small effect at 45-70 ℃. The sn-l,3-specificity of Lipozyme TI. IM is not satisfactory when reaction temperature is higher than 60 ℃. The optimal residence time and temperature are 30-40 min and 55 ℃, respectively. Among the solvents, including acetone, isopropanol, tert-butanol, and isobutanol, used to recover the activity of the used Lipozyme TL IM, acetone is the most suitable one than the other solvents.
