[Objective]The aim was to study homology between Ran gene in Allium cepa,Allium sativum and Brassica napus and Ran2 gene in Arabidopsis in order to determine whether three kinds of plant material as substitute for Arabidopsis. [Method]By using RT-PCR method,homology gene was cloned from totoal RNA which extracted from splinter cells of Allium cepa,Allium sativum and Brassica napus with Arabidopsis Ran2 primer,then,carrying out sequence and comparative analysis. [Result]The results showed that the open reading frames of Ran genes in Allium cepa,Allium sativum and Brassica napus were 666,663,666 bp,coding 221,220 and 221 amino acids respectively,with the molecular weight of 24.3 kDa. The sequence analysis showed that the amino acid homology of Ran genes between Allium cepa,Allium sativum,Brassica napus and Arabidopsis Ran2 were respectively 99.1 %,100 % （except an Asp D at Allium sativum C terminal）,96.4 %. The phylogenetic tree indicated that Ran genes from Allium cepa and Allium sativum had closer evolutionary relationship with Arabidopsis Ran2. [Conclusion]The research laid a foundation for further study on the biological function of plant Ran gene.
核内小分子GTP结合蛋白(a small nuclear GTP-binding protein,Ran)是一种分布于真核细胞核内含量十分丰富的小分子GTP酶,是Ras基因大家族中的一员。Ran具有多种生物学功能,参与调节核物质转运,调控核膜装配、纺锤体组装、DNA复制、RNA转录、加工和运输、细胞周期及LPS信号传导等。
