The effect of repressors on ion channel gene expression was studied. The hKv4.3 promoter and the sequence (+2—+160, S160) of 5′-UTR of the hKv4.3 gene was cloned into the pβ-gal-Basic vector. The transient expression of the pβ-gal vector and the analysis of the relative activity of %β%|galactosidase were carried out. The analysis of the mRNA level was carried out with the RT-PCR method. S160 could intensively repress the expression of the hKv4.3 gene with position-specificity. The level of mRNA did not alter obviously. A repressor(S160), in 5′-UTR of the hKv4.3 gene was found and its repression to gene expression may play a role in the post-transcription process.
LI HaoJIANG Chun-laiYU Xiang-huiWU Yong-geLI WeiKONG Wei
