Objective: 5-Aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly (adenosine 5’-diphosphate ribose) polymerase, plays an important role in the tissue injury associated with ischaemia-reperfusion injury and inflammation by inhibiting the activity of poly (adenosine 5’-diphosphate ribose) polymerase and the expression of cell adhesion molecules such as ICAM-1, P-selectin et al. But how about it in the tumor is not clear. The aim of the present study was to study the effects of 5-Aminoisoquinolinon on the adhesion of colon carcinoma line HT-29 cells to human umbilical vein endothelial cells; and the effects of 5-Aminoisoquinolinon on the expression of ICAM-1, P-selectin and the activity of poly (adenosine 5’-diphosphate ribose) polymerase in colon carcinoma HT-29 cells. Methods: The adhesion of HT-29 cells to human umbilical vein endothelial cells was detected by adhesive experiment. Immunocytochemically Streptavidin-Peroxidase method was used to investigate the expression of ICAM-1, P-selectin and Poly (adenosine 5’-diphosphate ribose)( the product of poly (adenosine 5’-diphosphate ribose) polymerase activation). Results: the results of the adhesion assay of HT-29 cells to HUVEC showed that the OD570 value in each 5-AIQ-treated group was significant lower than that in the control group (5-AIQ-untreated) in a dose-dependent manner. The expression of ICAM-1, P-selectin and Poly (adenosine 5’-diphosphate ribose) was significant lower in 5-Aminoisoquinolinone-treated HT-29 cell group than that in 5-Aminoisoquinolinone- untreated groups. Conclusion: The data suggest that 5-Aminoisoquinolinone can inhibit the adhesion of HT-29 cells to human umbilical vein endothelial cells. 5-Aminoisoquinolinone also can inhibit poly (adenosine 5’-diphosphate ribose) polymerase activation and the expressions of ICAM-1 and P-selectin in HT-29 cells. 5-Aminoisoquinolinone probably contributes to the prevention of tumor cell metastasis. Further study is needed.
Objective:To investigate the relationship of poly(ADP-ribose)glycohydrolase(PARG) with poly(ADP-ribose) polymerase(PARP),vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(b-FGF) in colorectal carcinoma(CRC).Methods:Immunohistochemical analysis was used to detect PARG,PARP,VEGF and b-FGF in human colorectal carcinoma.Flow cytometry was used to detect PARG and PARP in murine CT26 cell line.Gallotannin(GLTN) was served as PARG inhibitor.Results:The individual positive rates of PARG,PARP,VEGF and b-FGF were 55.81%(24/43),97.67%(42/43),79.07%(34/43) and 81.40%(35/43),respectively,which were significantly higher than those of control group.The positive PARG was correlated to PARP(r=0.3703,P<0.05) and b-FGF(r=0.4838,P<0.05).The positive PARP was correlated to VEGF(r=0.3968,P<0.05) and b-FGF(r=0.5610,P<0.05).Both PARG and PARP were expressed in CT26 cells.The positive staining rates of PARG and PARP in GLTN-treated group were 7.3% and 52.38%,respectively.They were markedly reduced than those of control group(55.41% and 95.28%,P<0.01,n=10000).Conclusion:The data suggest that PARG expression probably plays a role for VEGF and b-FGF expression in colorectal carcinoma.
